Hautriwaic acid as one of the hepatoprotective constituent of Dodonaea viscosa
Hautriwaic acid as one of the hepatoprotective constituent of Dodonaea viscosa
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DOI：
10.1016/j.phymed.2013.08.019
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发表时间：
2014-01-15
期刊：
影响因子：
7.9
通讯作者：
Nadeem, Said
中科院分区：
文献类型：
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作者：
Ali, Hamid;Kabir, Nurul;Nadeem, Said
众所周知，肝炎及其并发症如肝硬化或肝细胞癌是世界上主要的健康问题之一，特别是因为没有特定的治疗方法。在本研究中，我们研究了整个植物的Dodonaea viscossa及其乙酸乙酯，水，丁醇和正己烷馏分对四氯化碳（CCl4）诱导的大鼠肝毒性的甲醇提取物的肝保护潜力。肝保护作用通过CCl4损伤后血清酶（ALT、AST和ALP）的降低以及组织病理学和免疫组织化学进行评估。甲醇提取物降低血清酶水平（ALT，AST和ALP）下降到对照水平，尽管四氯化碳治疗。通过组织病理学评估，它还将CCl4诱导的损伤面积减少至0%。甲醇提取物还减少了中心静脉区域周围的CD68+巨噬细胞的数量。这些保肝作用优于阳性对照水飞蓟素。与阳性对照水飞蓟素相比，丁醇和正己烷级分显示ALT、AST和ALP水平升高。组织学显示，丁醇和正己烷级分对肝细胞的损伤类似于30%，与CCl4处理的对照相比，丁醇和正己烷级分仍显示出一定的保护活性。HPLC指纹图谱表明，存在于甲醇提取物及其乙酸乙酯，和水馏分中的hautriwaic酸可能是负责这种肝保护活性的Dodonaea viscossa，这是证实了在体内实验。(C)2013 Elsevier GmbH. All rights reserved.
It is widely known that hepatitis and its complications such as cirrhosis or hepatocellular carcinoma are one of the major health problems of the world especially since no specific treatment is available. In the present study we investigated the hepatoprotective potential of the methanolic extract of the whole plant of Dodonaea viscosa and its ethyl acetate, aqueous, butanol and n-hexane fractions against carbon tetrachloride (CCl4) induced hepatoxicity in rats. Hepatoprotection was assessed in terms of reduction in serum enzymes (ALT, AST, and ALP) that occur after CCl4 injury, and by histopathology and immunohistochemistry. The methanolic extract reduced the serum enzyme level (ALT, AST, and ALP) down to control levels despite CCl4 treatment. It also reduced the CCl4-induced damaged area to 0% as assessed by histopathology. The CD68+ macrophages were also reduced in number around the central vein area by the methanolic extract. These hepatoprotective effects were better than the positive control silymarin. Similar hepatoprotective activities were found with the ethyl acetate, and aqueous fractions of the methanolic extract The butanol and n-hexane fractions showed elevated levels of ALT, AST and ALP as compared to the positive control silymarin. Histopathology showed similar to 30% damage to the liver cells with the butanol and n-hexane fractions which still showed some protective activity compared to the CCl4 treated control. HPLC fingerprinting suggested that hautriwaic acid present in the methanolic extract and its ethyl acetate, and aqueous fractions may be responsible for this hepatoprotective activity of Dodonaea viscosa which was confirmed by in vivo experiments. (C) 2013 Elsevier GmbH. All rights reserved.