Matrix-Assisted Laser Desorption Ionization Imaging and Laser Ablation Sampling for Analysis of Fungicide Distribution in Apples
Matrix-Assisted Laser Desorption Ionization Imaging and Laser Ablation Sampling for Analysis of Fungicide Distribution in Apples
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DOI：
10.1021/acs.analchem.9b00566
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发表时间：
2019-05-07
影响因子：
7.4
通讯作者：
Murray, Kermit K.
中科院分区：
文献类型：
--
作者：
Pereira, Igor;Banstola, Bijay;Murray, Kermit K.
采用基质辅助激光解吸电离（MALDI）成像和红外（IR）激光烧蚀采样结合离线电喷雾电离质谱（ESI-MS）技术，测定了抑霉唑在苹果中的分布。使用MALDI图像来确定抑霉唑在其应用后长达7天的渗透深度。IR激光烧蚀取样和ESI-MS用于量化杀真菌剂的渗透速率，其被确定为每天约1 mm。在实验过程中，苹果皮中的抑霉唑浓度降低，7天后，在苹果内部6 mm处检测到0.015 ppm的杀真菌剂。大约60%的杀虫剂在7天后仍留在皮肤中。这项工作证明了MALDI成像的空间定位的杀菌剂在水果中结合红外激光消融和ESI-MS定量分析的效用。
A combination of matrix-assisted laser desorption ionization (MALDI) imaging and infrared (IR) laser ablation sampling with offline electrospray ionization mass spectrometry (ESI-MS) was used to determine the distribution of the fungicide imazalil in apples. MALDI images were used to determine the penetration depth of imazalil up to 7 days after its application. IR laser ablation sampling and ESI-MS were used to quantify the rate of penetration of the fungicide, which was determined to be approximately 1 mm per day. Imazalil concentration decreased in the apple skin over the course of the experiment, and after 7 days the fungicide was detected at 0.015 ppm 6 mm inside the apple. Approximately 60% of the pesticide remained in the skin after 7 days. This work demonstrates the utility of MALDI imaging for spatial localization of fungicide in fruit in combination with IR laser ablation and ESI-MS for quantitative analysis.