Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth
Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth
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DOI：
10.1016/j.joen.2008.04.009
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发表时间：
2008-08-01
影响因子：
4.2
通讯作者：
Noer, Jacques E.
中科院分区：
文献类型：
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作者：
Cordeiro, Mabel M.;Dong, Zhibong;Noer, Jacques E.
从人类脱皮乳牙(SHIP)中分离出干细胞，并鉴定为多能细胞。然而，目前尚不清楚SHIP能否在体内产生牙髓样组织。本研究的目的是评估种植在可生物降解支架中的组织的形态特征，将人牙片制备的支架移植到免疫缺陷小鼠体内。我们观察到，所形成的组织呈现出与生理牙髓非常相似的结构和细胞密度。通过对牙本质唾液蛋白的超微结构观察和免疫组织化学分析，证实其在体内可分化为成牙本质细胞样细胞。值得注意的是，SHADE还分化为内皮样细胞，这一点通过B-半乳糖苷酶染色显示，在组织工程中，含有血管的血管壁上的细胞被稳定地转导到LacZ。这项工作表明，脱落的乳牙构成了牙髓组织工程干细胞的一个可行来源。
Stem cells from human exfoliated deciduous teeth (SHED) have been isolated and characterized as multi-potent cells. However, it is not known whether SHED can generate a dental pulp-like tissue in vivo. The purpose of this study was to evaluate morphologic characteristics of the tissue formed when SHED seeded in biodegradable scaffolds prepared within human tooth slices are transplanted into immunodeficient mice. We observed that the resulting tissue presented architecture and cellularity that closely resemble those of a physiologic dental pulp. Ultrastructural analysis with transmission electron microscopy and immunohistochemistry for dentin sialoprotein suggested that SHED differentiated into odontoblast-like cells in vivo. Notably, SHED also differentiated into endothelial-like cells, as demonstrated by B-galactosidase staining of cells lining the walls of blood-containing vessels in tissues engineered with SHED stably transduced with LacZ. This work suggests that exfoliated deciduous teeth constitute a viable source of stem cells for dental pulp tissue engineering.