Electrical Stimulation Induces Calcium-Dependent Release of NGF From Cultured Schwann Cells
Electrical Stimulation Induces Calcium-Dependent Release of NGF From Cultured Schwann Cells
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电刺激诱导培养雪旺细胞钙依赖性 NGF 释放
DOI：
10.1002/glia.20951
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发表时间：
2010-04-01
期刊：
影响因子：
6.2
通讯作者：
Luo, Zhuojing
中科院分区：
文献类型：
--
作者：
Huang, Jinghui;Ye, Zhengxu;Luo, Zhuojing
周围神经损伤后，如果轴突在缝合部位交错再生，远端残端雪旺细胞（SCs）的神经生长因子（NGF）的产生会逐渐下降，这可能是限制神经损伤修复结果的一个重要因素。到目前为止。大量的研究致力于调节培养SCs中NGF的产生，但收效甚微。在本体外研究中，我们尝试电刺激（ES）刺激培养的SCs释放NGF，我们的数据表明，ES能够促进培养的SCs释放NGF。电场（1 Hz, 5 V/cm）使培养的SCs释放的NGF增加了41倍，ES诱导的NGF释放是钙依赖性的，细胞外或/和细胞内钙的消耗部分/完全消除了ES诱导的NGF释放。进一步的药理学干预表明，ES通过t型电压门控钙通道诱导钙内流，并从1,4,5 -三磷酸敏感储存和咖啡因/红嘌呤敏感储存中动员钙，这两者都有助于ES诱导的NGF释放增强。此外，ES诱导的NGF释放涉及钙触发的胞外分泌机制。这些发现表明，使用F-S刺激sc释放NGF是可行的。它通过促进受损神经的存活和生长，在促进神经再生方面具有很大的潜力，在神经损伤修复和神经组织工程中具有重要意义。(C) 2009 Wiley-Liss。公司。
Production of nerve growth factor (NGF) from Schwann Cells (SCs) progressively declines in the distal stump if axonal regeneration is staggered across the suture site after peripheral nerve injuries This may be an important factor limiting the Outcome of nerve injury repair. Thus far. extensive efforts are devoted to modulating NGF production in Cultured SCs, but little has been achieved. In the present in vitro study, electrical stimulation (ES) was attempted to stimulate cultured SCs to release NGF Our data showed that ES was capable of enhancing NGF release from cultured SCs An electrical field (1 Hz, 5 V/cm) caused a 4 1-fold increase in NGF release from cultured SCs The ES-induced NGF release is calcium dependent Depletion of extracellular or/and intracellular calcium partially/completely abolished the ES-induced NGF release. Further pharmacological interventions showed that ES Induces calcium influx through T-type voltage-gated calcium channels and mobilizes calcium from 1, 4, 5-trisphosphate-sensitive stores and caffeine/ryanodine-sensitive stores, both of which contributed to the enhanced NGF release induced by ES In addition, a calcium-triggered exocytosis mechanism was involved in the ES-induced NGF release from Cultured SCs These findings show the feasibility of using F-S in stimulating SCs to release NGF, which holds great potential in promoting nerve regeneration by enhancing Survival and outgrowth of damaged nerves, kind is of great significance In nerve injury repair and neuronal tissue engineering. (C) 2009 Wiley-Liss. Inc.