Adaptation of light-harvesting and energy-transfer processes of a diatom Chaetoceros gracilis to different light qualities
Adaptation of light-harvesting and energy-transfer processes of a diatom Chaetoceros gracilis to different light qualities
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DOI：
10.1007/s11120-020-00713-2
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发表时间：
2020-01-22
影响因子：
3.7
通讯作者：
Nagao, Ryo
中科院分区：
文献类型：
--
作者：
Akimoto, Seiji;Ueno, Yoshifumi;Nagao, Ryo
硅藻是海洋和淡水环境中微藻的主要类群。为了利用蓝色到绿色区域的光能，硅藻具有独特的天线色素蛋白复合物，岩藻黄素叶绿素a/c结合蛋白（fcp）。根据光的质量和数量，硅藻形成具有不同能量的fcp：普通型和红移型fcp。在本研究中，我们通过时间分辨荧光光谱研究了使用白色和单色发光二极管（led）生长的硅藻薄毛角毛藻细胞的光收集和能量转移过程的变化。由fcp收集的蓝色LED改变了涉及CP47的能量转移，抑制了向PSI的能量转移。在FCP和ps都被吸收的红色led条件下，向PSI的能量传递增强，FCP出现红移。红移的FCP在绿色和黄色led下也被识别出来，这表明缺乏波长较短的光导致了红移的FCP。讨论了红移fcp的函数。
Diatoms are a major group of microalgae in marine and freshwater environments. To utilize the light energy in blue to green region, diatoms possess unique antenna pigment-protein complexes, fucoxanthin chlorophyll a/c-binding proteins (FCPs). Depending on light qualities and quantities, diatoms form FCPs with different energies: normal-type and red-shifted FCPs. In the present study, we examined changes in light-harvesting and energy-transfer processes of a diatom Chaetoceros gracilis cells grown using white- and single-colored light-emitting diodes (LEDs), by means of time-resolved fluorescence spectroscopy. The blue LED, which is harvested by FCPs, modified energy transfer involving CP47, and suppressed energy transfer to PSI. Under the red-LED conditions, which is absorbed by both FCPs and PSs, energy transfer to PSI was enhanced, and the red-shifted FCP appeared. The red-shifted FCP was also recognized under the green- and yellow-LEDs, suggesting that lack of the shorter-wavelength light induces the red-shifted FCP. Functions of the red-shifted FCPs are discussed.