Both MicroRNA-155 and Virus-Encoded MiR-155 Ortholog Regulate TLR3 Expression.
Both MicroRNA-155 and Virus-Encoded MiR-155 Ortholog Regulate TLR3 Expression.
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MicroRNA-155 和病毒编码的 MiR-155 直向同源物均可调节 TLR3 表达
DOI：
10.1371/journal.pone.0126012
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qian K
中科院分区：
文献类型：
--
作者：
Hu X;Ye J;Qin A;Zou H;Shao H;Qian K
microRNA-155（miR-155）是TLR 3信号转导的重要调控因子。然而，miR-155和TLR 3之间的相互作用知之甚少。在此，我们重点研究了miR-155与TLR 3之间关系的调控。序列分析和萤火虫荧光素酶报告基因检测结果表明，miR-155靶基因存在于TLR 3的编码序列（CDS）中。miR-155模拟物或病毒编码的同源物可抑制TLR 3蛋白在鸡胚成纤维细胞中的表达。值得注意的是，内源性miR-155诱导对TLR 2、4和7配体刺激的禽类巨噬细胞系HD 11细胞中TLR 3表达出现负调控。此外，用miR-155阿戈米尔处理增加了TLR 3水平，而用miR-155阿戈米尔显著降低了TLR 3的丰度。另外，miR-155可能通过TLR 3信号通路抑制IFN-β的产生。所有这些发现可能揭示了miR-155调节TLR 3免疫反应的新机制。
MicroRNA-155 (miR-155) has been as an important controller of TLR3 signalling. However, the interactions between miR-155 and TLR3 are poorly understood. Here, we focused on the regulation of the relationship between miR-155 and TLR3. Sequence analyses and firefly luciferase reporter assay revealed that miR-155 target were present in the coding sequences (CDS) of TLR3. And the expression of the TLR3 protein could be inhibited by a miR-155 mimic or by a virally encoded orthologue in chick embryo fibroblast cells. Notably, endogenous miR-155 induction emerged a negative regulation on TLR3 expression in TLR2, 4 and 7 ligands stimulated HD11 cells, an avian macrophage cell line. Moreover, treatment with the miR-155 antagomir increased TLR3 levels while significantly decreased the abundance of TLR3 with miR-155 agomir. In addition, our data showed that miR-155 could inhibit IFN-β production possibly though TLR3 signal pathway. All these findings might reveal a new mechanism by which miR-155 can regulate the TLR3 immune response.