Nuclear magnetic resonance assignment of the vinyl hydrogens of phosphoenolpyruvate. Stereochemistry of the enolase reaction.
Nuclear magnetic resonance assignment of the vinyl hydrogens of phosphoenolpyruvate. Stereochemistry of the enolase reaction.
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磷酸烯醇丙酮酸的乙烯基氢的核磁共振分配。
DOI：
10.1021/ja00716a044
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发表时间：
1970
影响因子：
15
通讯作者：
I. A. Rose
中科院分区：
文献类型：
--
作者：
M. Cohn;J. Pearson;E. O'Connell;I. A. Rose
通过对磷酸烯醇式丙酮酸和磷酸烯醇式丙酮酸-L-13C的核磁共振谱的分析，得出结论：前场质子代表反式到磷酸基团的位置。由(2R，3R)-2-磷酸甘油酸酯-3-d与烯醇化酶反应生成的磷酸烯醇式丙酮酸-3-d仅在该位置含有氢。因此，消除水分是特别不利的。烯醇酶(EC：4.2.1.1 1，2-磷酸-~-甘油水解酶)催化2PGA3和PEP3的可逆相互转化(反应1)。
From an analysis of the nmr spectra of phosphoenolpyruvate and phosphoenolpyruvate-l-13C it is concluded that the downfield proton represents the position trans to the phosphate group. The phosphoenolpyruvate-3-d formed from (2R,3R)-2-phosphoglycerate-3-d by reaction with enolase is found to contain deuterium only in this position. Thus the elimination of water is specifically anti. nolase (EC: 4.2.1.1 1, 2-phospho-~-glycerate hyE drolyase) catalyzes the reversible interconversion of 2PGA3 and PEP3 (reaction 1).