The absence of differentiation-promoting response of astroglioma cells to glia maturation factor
The absence of differentiation-promoting response of astroglioma cells to glia maturation factor
复制标题
星形胶质瘤细胞对神经胶质成熟因子缺乏促进分化的反应
DOI：
10.1016/0006-8993(84)90405-0
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Kyoko Kana
中科院分区：
文献类型：
--
作者：
Taiji Kato;J. Ito;K. Ishikawa;Kayo Mizutani;R. Tanaka;S. Wakabayashi;I. Horiuchi;Kanefusa Kato;Kyoko Kana
本实验用3种星形胶质瘤细胞（GE-12、C6和GA-1）、神经鞘瘤样细胞（354 A）和混合胶质瘤细胞（LRM-55）研究了胶质成熟因子（GMF）对细胞增殖和分化的影响。在指数生长期，所有胶质瘤细胞的生长速率均被GMF增强，无论是否存在血清，但该因子未能使饱和密度超过在无GMF的培养基中观察到的对照水平，即使在化学成分确定的培养基（N2培养基）中。GMF能显著降低神经鞘瘤样细胞在N2培养基中的饱和密度。GMF可使神经鞘瘤样细胞内S-100蛋白含量增加10倍，2′，3 ′-环核苷酸磷酸水解酶活性增加1.5倍，而使星形胶质瘤细胞内S-100蛋白含量和磷酸甘油脱氢酶活性降低。所有星形胶质细胞瘤细胞都向培养基中分泌大量的生长促进因子，这些生长促进因子具有与GMF相似的化学性质，并刺激正常成胶质细胞的增殖;但神经鞘瘤样细胞没有，尽管它们产生少量这样的因子。这些发现意味着星形胶质细胞瘤细胞被剥夺了对GMF的分化促进反应，而神经鞘瘤样细胞除了对GMF的增殖反应外，仍然保留了对GMF的反应。
The effects of glia maturation factor (GMF) on cell proliferation and differentiation were investigated with 3 astroglioma cells (GE-12, C6, and GA-1), Schwannoma-like cells (354A), and mixed glioma cells (LRM-55). In the exponentially growing phase the growth rates of all glioma cells were enhanced by GMF regardless of the presence or absence of serum, but the factor failed to make the saturation density surpass the control level observed in the medium without GMF even in the chemically defined medium (N2 medium). GMF markedly lowered the saturation density of Schwannoma-like cells in N2 medium. Although GMF increased the intracellular content of S-100 protein 10-fold and 2′,3′-cyclic nucleotide phosphohydrolase activity 1.5-fold in Schwannoma-like cells, GMF conversely decreased the S-100 contents and glycerol phosphate dehydrogenase activity in astroglioma cells. All the astroglioma cells secreted into the culture medium large quantities of a growth-promoting factor(s) which had similar chemical properties to those of GMF and stimulated the proliferation of normal glioblasts; but Schwannoma-like cells did not, although they produced a small amount of such a factor(s). These findings imply that astroglioma cells are deprived of the differentiation-promoting response to GMF while Schwannoma-like cells still preserve the response in addition to the proliferative response to GMF.