Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions
Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions
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DOI：
10.1007/978-1-4939-9167-9_21
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Coutermarsh-Ott, Sheryl
中科院分区：
文献类型：
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作者：
Coutermarsh-Ott, Sheryl
弓形虫是一种细胞内顶复门寄生虫，对人类和动物健康都非常重要。目前的研究已经确定了寄生虫特有的各种重要且必要的因素，使其能够感染并在多种哺乳动物宿主中持续存在。然而，为了继续加深我们对弓形虫发病机制的了解，这些寄生虫特征的相关性需要在动物模型中继续研究。在下一章中，我们提出了 C57BL/6 小鼠腹膜内感染弓形虫速殖子的模型，该模型在 C57BL/6 小鼠中引发强烈的急性免疫反应。此外，我们提出了对腹膜和支气管肺泡灌洗液进行采样和分析的方法，以评估对寄生虫的局部和全身免疫反应。
Toxoplasma gondii is an intracellular, apicomplexan parasite of great importance in both human and animal health. Current research has identified a variety of important and necessary factors specific to the parasite that enable it to infect and persist in a wide array of mammalian hosts. However, in order to continue to build our understanding of T. gondii pathogenesis, the relevance of these parasite characteristics needs continued investigation in animal models. In the following chapter, we present a model of intraperitoneal infection of C57BL/6 mice with T. gondii tachyzoites that, in C57BL/6 mice, elicits a strong acute immune response. Moreover, we present methods for sampling and analyzing peritoneal and bronchoalveolar lavage fluids in order to assess localized and systemic immune reactions to the parasite.