Gonadotrophin and steroid levels around the time of first ovulation in the rat.
Gonadotrophin and steroid levels around the time of first ovulation in the rat.
复制标题
大鼠第一次排卵时的促性腺激素和类固醇水平。
作者：
H. Meijs;J. Uilenbroek;W. D. de Greef;F. de Jong;P. Kramer
采用放射免疫法测定雌性大鼠第一次排卵前7天（第一次排卵日为第0天）至第二次排卵后1天血液中黄体生成素、卵泡刺激素、催乳素、雌二醇和黄体酮的浓度。从第7天到第2天，血清LH浓度在29 ~ 59 ng niamdd -大鼠LH RP-1/ml之间变化。在第一次排卵的前一天（第1天），血清LH在13.00至15.00 h之间急剧上升，在17.00 h达到最大值2100 ng/ml，此后LH水平下降到不可检测的值（低于15 ng/ml）。第-7天至第2天血清FSH浓度在108-154 ng /ml之间变化；浓度在第1天下午和第1次发情后的早晨均有所升高，在第1天19.00 h时达到最高值1150 ng/ml。在第二次排卵前一天（第4天）LH和FSH再次出现峰值。第一次排卵前几天催乳素浓度普遍较低。在接近排卵期时出现小幅度升高（12-7 ng/ml，第6天；23-9 ng/ml，第2天）。在第1天，15.00 h出现峰值（118 ng/ml），与第4天的成体发情大鼠相似。血浆孕酮浓度也在第1天17.00 h达到峰值（50 ng/ml），第6天至第2天的浓度在3-5 ng/ml之间。第一次排卵后的黄体酮浓度显示出成人周期的特征。从-6天到-2天，血浆雌二醇水平未检测到（低于10 pg/ml）；雌二醇浓度在第1天明显升高，达到98 pg/ml。在第二次排卵前1天和2天，雌二醇水平再次上升。讨论了青春期和成人促排卵激素峰值的异同。
Luteinizing hormone, FSH, prolactin, oestradiol and progesterone concentrations were measured by radioimmunoassay in the blood of female rats from 7 days before the first ovulation (the day of first ovulation is designated day 0) until the day after the second ovulation. Serum LH concentrations varied between 29 and 59 ng NIAMDD-rat-LH RP-1/ml from days -7 to -2. On the day preceding first ovulation (day -1) a steep increase in serum LH was found between 13.00 h and 15.00 h reaching a maximal value of 2100 ng/ml at 17.00 h. Thereafter LH levels decreased to undetectable values (less than 15 ng/ml). Serum FSH concentrations from days -7 to -2 varied between 108-154 ng NIAMDD-rat-FSH RP-1/ml; increased concentrations were found on the afternoon of day -1 and on the morning thereafter (first oestrus) with a maximal value of 1150 ng/ml at 19.00 h on day -1. On the day preceding the second ovulation (day +4) LH and FSH peaks were again found. Prolactin concentrations on the days preceding first ovulation were generally low. A small rise was found nearing first ovulation (12-7 ng/ml, day -6; 23-9 ng/ml, day -2). On day -1 a peak (118 ng/ml) was found at 15.00 h, similar to that found on day +4 in adult pro-oestrous rats. Plasma progesterone concentrations also reached a peak value (50 ng/ml) on day -1, at 17.00 h, preceded by concentrations between 3-5 ng/ml from days -6 to -2. Progesterone concentrations after the first ovulation showed a pattern characteristic of the adult cycle. Plasma oestradiol levels were un-etectable (less than 10 pg/ml) from days -6 to -2; a clearly increased oestradiol concentration was found on day -1, reaching a value of 98 pg/ml. One and two days before the second ovulation, oestradiol levels rose again. The differences and similarities between pubertal and adult pro-oestrous hormone peaks are discussed.