Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers
Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers
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DOI：
10.1016/s0167-7012(99)00136-0
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发表时间：
2000-03-01
影响因子：
2.2
通讯作者：
Gray, KM
中科院分区：
文献类型：
--
作者：
Blosser, RS;Gray, KM
脂肪酰高丝氨酸内酯(AHLS)被多种革兰氏阴性菌用作细胞外群体感应信号。通过激活属于LuxR转录调节家族的蛋白质，这些信号代谢物允许物种内依赖于种群密度的基因调控，以及不同细菌之间的物种间交流。AHLS的实验检测对于鉴定细菌的群体感应能力是重要的。紫罗兰色杆菌是一种革兰氏阴性细菌，能在AHL N-己酰高丝氨酸内酯(C6HSL)存在时产生紫色色素紫罗兰素。Mini-Tn5突变株C。Viaceum CV0blu缺乏产生这种信号分子的能力，但在C6HSL和其他多种短链AHL的存在下仍具有合成紫罗兰素的能力。我们已经开发了一种定量生物测定方法，可以测量该菌株在不同浓度的AHL分子存在时产生的紫罗兰素的量。这种新的检测方法提供了一种量化细菌培养中存在的特定AHL数量的方法，并可用于测量不同菌株或给定物种的不同批次培养物之间AHL产量的差异。(C)2000 Elsevier Science B.V.保留所有权利。
Fatty acyl homoserine lactones (AHLs) are used as extracellular quorum sensing signals by a variety of Gram-negative bacteria. By activating proteins belonging to the LuxR family of transcriptional regulators, these signal metabolites allow population density-dependent gene regulation within a species, as well as interspecies communication among different bacteria. The experimental detection of AHLs is important in the identification of quorum sensing capabilities in bacteria. Chromobacterium violaceum is a Gram-negative bacterium that produces the purple pigment violacein in response to the presence of the AHL N-hexanoyl homoserine lactone (C6HSL). The mini-Tn5 mutant strain C. violaceum CV0blu is deficient in the production of this signal molecule but retains the ability to synthesize violacein in response to the presence of C6HSL and a variety of other short-chain AHLs. We have developed a quantitative bioassay that measures the amount of violacein produced by this strain in response to the presence of different concentrations of various AHL molecules. This new assay provides a means of quantifying the amount of a given AHL present in a bacterial culture and can be used to measure differences in AHL production among different strains or different batch cultures of a given species. (C) 2000 Elsevier Science B.V. All rights reserved.