Quantum dot-based lab-on-a-bead system for multiplexed detection of free and total prostate-specific antigens in clinical human serum samples
Quantum dot-based lab-on-a-bead system for multiplexed detection of free and total prostate-specific antigens in clinical human serum samples
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DOI：
10.1016/j.nano.2015.03.003
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发表时间：
2015-07-01
影响因子：
5.4
通讯作者：
Sukhanova, Alyona
中科院分区：
文献类型：
--
作者：
Brazhnik, Kristina;Sokolova, Zinaida;Sukhanova, Alyona
基于量子点编码的微珠的免疫诊断实验室-微珠悬浮微阵列已被开发并临床前验证用于人血清样品中前列腺癌标志物的多重定量检测。该芯片的灵敏度和特异性与“金标准”单分析物ELISA相似。此外，该阵列具有改进的免疫测定能力，确保多种癌症生物标志物的定量检测，并且可以在相当宽的浓度动态范围内操作。该阵列的特点是减少了分析的时间和成本，并与经典的流式细胞仪兼容。概念验证临床前试验确保同时定量测定人血清中的游离和总前列腺特异性抗原，并明确区分对照和临床样品。所提出的方法是灵活的，并铺平了道路，以发展各种各样的免疫诊断检测多种多样的早期诊断的各种疾病。从临床编辑：癌症的早期诊断可以导致更好的预后为患者。因此，特异性肿瘤标志物的使用在临床实践中被广泛采用。传统的筛选方法仅采用单一标记。作者在这里开发了一种基于量子点编码微珠的微阵列系统，可用于多重定量检测。对患者样本的验证结果应该会导致开发更广泛的其他疾病检测方法。(C)2015 Elsevier Inc. All rights reserved.
An immunodiagnostic lab-on-a-bead suspension microarray based on microbeads encoded with quantum dots (QDs) has been developed and preclinically validated for multiplexed quantitative detection of prostate cancer markers in human serum samples. The sensitivity and specificity of the microarray are similar to those of "gold-standard" single-analyte ELISA. Moreover, the array has an improved immunoassay capacity, ensures quantitative detection of multiple cancer biomarkers and may be operational in a considerably wider dynamic range of concentrations. The array is characterized by reduced time and cost of analysis and is compatible with classical flow cytometers. Proof-of-concept preclinical tests ensured simultaneous quantitative determination of free and total prostate-specific antigens in human serum, with clear discrimination between the control and clinical samples. The proposed approach is flexible and paves the way to development of a wide variety of immunodiagnostic assays for multiplexed early diagnosis of various diseases.From the Clinical Editor: Early diagnosis of cancer can result in better prognosis for patients. Thus, the use of specific tumor markers is widely employed in clinical practice. Traditional screening methods only employ single markers. The authors here developed a microarray system based on microbeads encoded with quantum dots (QDs), which can be used for multiplexed quantitative detection. The validated results on patient samples should lead to the development of a wider variety of assays for other diseases. (C) 2015 Elsevier Inc. All rights reserved.