Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of Ta APETALA3
Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of Ta APETALA3
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小麦 AGAMOUS LIKE 6 转录因子通过调节 Ta APETALA3 的表达在雄蕊发育中发挥作用
DOI：
10.1242/dev.177527
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发表时间：
2019-10-01
期刊：
影响因子：
4.6
通讯作者：
Li, Haifeng
中科院分区：
文献类型：
--
作者：
Su, Yali;Liu, Jinxing;Li, Haifeng
摘要 先前的研究揭示了水稻和玉米 AGAMOUS LIKE 6 (AGL6) 基因 OsMADS6 和 ZAG3 分别在花发育中的功能。然而，三个小麦（Triticum aestivum）AGL6 基因的功能仍不清楚。在此，我们报道了小麦AGL6同源基因在雄蕊发育中的主要功能。在RNAi植物中，雄蕊在数量和形态上表现出异常，并且有转变为心皮的倾向。一致的是，B 类基因 TaAPETALA3 (AP3) 和生长素响应基因 TaMGH3 的表达下调，而水稻心皮识别基因 DROOPING LEAF 的小麦直系同源基因在 RNAi 雄蕊中异位表达。 TaAGL6 蛋白直接结合 TaAP3 的启动子。酵母单杂交和瞬时表达测定进一步表明TaAGL6在体内正向调节TaAP3的表达。小麦 AGL6 转录因子与 TaAP3、TaAGAMOUS 和 TaMADS13 相互作用。我们的研究结果表明，TaAGL6 转录因子通过 TaAP3 和其他相关基因的转录调控在雄蕊发育中发挥重要作用。我们提出了一个模型来说明这种调节的功能和可能的机制。这项研究扩展了我们对 AGL6 基因的理解。摘要：在小麦中，AGAMOUS LIKE 6 基因作为 E 类基因发挥作用，通过 Ta APETALA3 的转录调控在雄蕊发育中发挥重要作用。
ABSTRACT Previous studies have revealed the functions of rice and maize AGAMOUS LIKE 6 (AGL6) genes OsMADS6 and ZAG3, respectively, in floral development; however, the functions of three wheat (Triticum aestivum) AGL6 genes are still unclear. Here, we report the main functions of wheat AGL6 homoeologous genes in stamen development. In RNAi plants, stamens showed abnormality in number and morphology, and a tendency to transform into carpels. Consistently, the expression of the B-class gene TaAPETALA3 (AP3) and the auxin-responsive gene TaMGH3 was downregulated, whereas the wheat ortholog of the rice carpel identity gene DROOPING LEAF was ectopically expressed in RNAi stamens. TaAGL6 proteins bind to the promoter of TaAP3 directly. Yeast one-hybrid and transient expression assays further showed that TaAGL6 positively regulates the expression of TaAP3 in vivo. Wheat AGL6 transcription factors interact with TaAP3, TaAGAMOUS and TaMADS13. Our findings indicate that TaAGL6 transcription factors play an essential role in stamen development through transcriptional regulation of TaAP3 and other related genes. We propose a model to illustrate the function and probable mechanism of this regulation. This study extends our understanding of AGL6 genes. Summary: In wheat, AGAMOUS LIKE 6 genes function as E-class genes and play essential roles in stamen development through transcriptional regulation of Ta APETALA3.