DELETION OF THE MATING-TYPE SEQUENCES IN PODOSPORA-ANSERINA ABOLISHES MATING WITHOUT AFFECTING VEGETATIVE FUNCTIONS AND SEXUAL-DIFFERENTIATION
DELETION OF THE MATING-TYPE SEQUENCES IN PODOSPORA-ANSERINA ABOLISHES MATING WITHOUT AFFECTING VEGETATIVE FUNCTIONS AND SEXUAL-DIFFERENTIATION
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DOI：
10.1007/bf00284694
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发表时间：
1993-11-01
期刊：
影响因子：
--
通讯作者：
PICARD, M
中科院分区：
文献类型：
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作者：
COPPIN, E;ARNAISE, S;PICARD, M
柄孢壳菌（Podospora anserina）的交配型位点控制性细胞的融合以及子实体发育的后续阶段。该位点的两个等位基因由特定的DNA区域所界定，mat +为3.8 kb，mat -为4.7 kb，它们具有相同的侧翼序列。在此我们对几个缺失了mat +特异性序列的突变体进行了特性描述。一个突变体是偶然获得的，另外两个是通过基因替换构建的。这些突变体与任何一种mat基因型的菌株交配都存在缺陷，但仍然能够分化出有性生殖结构。交配型的缺失在营养生长过程中不会导致任何可察觉的表型：特别是它不会改变菌株的寿命。如果用含有完整mat +或mat -信息的DNA对突变体进行转化，它们可以恢复交配能力。这些转化体在杂交中的行为与参照的mat +或mat -菌株相同，这表明转基因的mat +和mat -即使整合在异位位点也是完全有功能的。
The mating-type locus of Podospora anserina controls fusion of sexual cells as well as subsequent stages of development of the fruiting bodies. The two alleles at the locus are defined by specific DNA regions comprising 3.8 kb for mat + and 4.7 kb for mat -, which have identical flanking sequences. Here we present the characterization of several mutants that have lost mat + -specific sequences. One mutant was obtained fortuitously and the other two were constructed by gene replacement. The mutants are deficient in mating with strains of either mat genotype but are still able to differentiate sexual reproductive structures. The loss of the mating type does not lead to any discernible phenotype during vegetative growth: in particular it does not change the life span of the strain. The mutants can recover mating ability if they are transformed with DNA containing the complete mat + or mat - information. The transformants behave in crosses as do the reference mat + or mat - strains, thus indicating that the transgenic mat + and mat - are fully functional even when they have integrated at ectopic sites.