Basal forebrain GABAergic neurons promote arousal and predatory hunting
Basal forebrain GABAergic neurons promote arousal and predatory hunting
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基底前脑 GABA 能神经元促进唤醒和掠夺性狩猎
DOI：
10.1016/j.neuropharm.2020.108299
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发表时间：
2020-12-01
影响因子：
4.7
通讯作者：
Yu, Chang-Xi
中科院分区：
文献类型：
--
作者：
Cai, Ping;Chen, Li;Yu, Chang-Xi
掠夺性捕猎是动物获取有价值的营养和能量的一种重要方式，这在很大程度上依赖于高度的觉醒状态。然而，掠夺性捕猎背后的神经基础在很大程度上仍未明确。在此，我们报道基底前脑（BF）的γ-氨基丁酸（GABA）能神经元在调节掠夺性捕猎中起重要作用。我们的研究结果表明，在小鼠捕食猎物（蟋蟀）和进食食物过程中，BF的GABA能神经元被激活。对BF的GABA能神经元进行光遗传学激活，会对人工和天然猎物立即引发类似掠夺的行为，显著缩短攻击潜伏期，同时增加攻击概率以及被杀死的天然猎物（蟋蟀）数量。与激活BF的GABA能神经元胞体的效果类似，对其在腹侧被盖区（VTA）的轴突末梢进行光激活也强烈促进掠夺性捕猎。此外，对GABA能的BF - VTA通路进行光激活显著增加了小鼠对各种食物的摄取量。通过对脑电图和肌电图的同步记录，我们发现对GABA能的BF - VTA通路进行光激活会引起即时觉醒并维持长期清醒。总之，我们的研究结果清楚地表明，GABA能的BF是掠夺性捕猎的一个关键神经基础，并通过GABA能的BF - VTA通路促进这种行为。
Predatory hunting is an important approach for animals to obtain valuable nutrition and energy, which critically depends on heightened arousal. Yet the neural substrates underlying predatory hunting remain largely undefined. Here, we report that basal forebrain (BF) GABAergic neurons play an important role in regulating predatory hunting. Our results showed that BF GABAergic neurons were activated during the prey (cricket)-hunting and food feeding in mice. Optogenetic activation of BF GABAergic neurons evoked immediate predatory-like actions to both artificial and natural preys, significantly reducing the attack latency while increasing the attack probability and the number of killed natural prey (crickets). Similar to the effect of activating the soma of BF GABAergic neurons, photoactivation of their terminals in the ventral tegmental area (VTA) also strongly promotes predatory hunting. Moreover, photoactivation of GABAergic BF VTA pathway significantly increases the intake of various food in mice. By synchronous recording of electroencephalogram and electromyogram, we showed that photoactivation of GABAergic BF VTA pathway induces instant arousal and maintains long-term wakefulness. In summary, our results clearly demonstrated that the GABAergic BF is a key neural substrate for predatory hunting, and promotes this behavior through GABAergic BF VTA pathway.Y