SPERM-ENGULFING RESPONSE OF SEA-URCHIN EGG SURFACES INSEMINATED WITH ACROSOME-REACTED STARFISH SPERM
SPERM-ENGULFING RESPONSE OF SEA-URCHIN EGG SURFACES INSEMINATED WITH ACROSOME-REACTED STARFISH SPERM
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DOI：
10.1002/mrd.1120210207
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发表时间：
1988-10-01
期刊：
影响因子：
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通讯作者：
OSANAI, K
中科院分区：
文献类型：
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作者：
KYOZUKA, K;OSANAI, K
精卵相互作用进行了研究，在两个类间组合的海胆（球海胆nudus和Hemicentrotus pulcherrimus）的鸡蛋和海星（Asterina pectinifera和Asterias amurensis）精子。这些组合之间的异花受精是不成功的。当无卵黄膜的海胆卵与顶体反应的海星精子混合时，卵表面细长的微绒毛包裹着精子头部。这种在裸露卵表面上观察到的精子吞噬反应仅在顶体反应开始后立即在精子中诱导。进一步的受精事件，如配子膜融合或皮质颗粒的放电，没有进行。这些观察结果表明，顶体反应的精子可以诱导异源卵表面的局部反应，这是独立的配子膜融合和卵激活。
Sperm-egg interaction was examined in two interclass combinations of sea urchin (Strongylocentrotus nudus and Hemicentrotus pulcherrimus) eggs and starfish (Asterina pectinifera and Asterias amurensis) sperm. Cross-fertilization was unsuccessful between these combinations. When the vitelline coat-free sea urchin eggs were mixed with acrosome-reacted starfish sperm, the elongated microvilli on the egg surface wrapped the sperm head. This sperm-engulfing response observed on the denuded egg surface was induced only in sperm immediately after initiation of the acrosome reaction. Further fertilization events, such as gamete membrane fusion or discharge of cortical granules, did not proceed. These observations suggest that acrosome-reacted sperm can induce a local response on the heterologous egg surface, that is independent of gamete membrane fusion and egg activation.