MESODERMAL INDUCTION IN EARLY AMPHIBIAN EMBRYOS BY ACTIVIN-A (ERYTHROID-DIFFERENTIATION FACTOR)
MESODERMAL INDUCTION IN EARLY AMPHIBIAN EMBRYOS BY ACTIVIN-A (ERYTHROID-DIFFERENTIATION FACTOR)
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DOI：
10.1007/bf00383771
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
UENO, N
中科院分区：
文献类型：
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作者：
ASASHIMA, M;NAKANO, H;UENO, N
最近，转化生长因子β的中胚层诱导作用。 (TGF-β)蛋白家族已被广泛研究。为了阐明这些蛋白质的功能，对猪抑制素 A 和激活素 A（红细胞分化因子；EDF）进行了检查。用激活素 A 处理外植体导致中胚层衍生物的分化，如间充质、脊索、血细胞和肌肉，但抑制素 A 的作用要小得多。通过使用针对肌肉肌球蛋白的多克隆抗体进行分析，还证实了激活素 A 诱导的中胚层分化。通过间接免疫荧光分析，在激活素 A 处理的外植体中观察到肌肉块的分化，而在抑制素 A 处理和对照外植体中未观察到分化。这些发现证实了TGF-β的这种蛋白。家族具有中胚层诱导能力。
Recently the mesoderm-inducing effects of the transforming growth factor .beta. (TGF-.beta.) family of proteins have been widely examined. In an attempt to elucidate the functions of these proteins, porcine inhibin A and activin A (erythroid differentiation factor; EDF) were examined. Treatment of explants with activin A led to differentiation of mesodermal derivatives such as mesenchyme, notochord, blood cells and muscle, but inhibin A had a much lesser effect. The mesodermal differentiation induced by activin A was also confirmed by analyses using a polyclonal antibody against muscle myosin. By indirect immunofluorescence analysis, the differentiation of muscle blocks was observed in the activin-A-treated explants, whereas no differentiation was observed in inhibin-A-treated and control explants. These findings confirm that this protein of the TGF-.beta. family has mesoderm-inducing ability.