Coiled-coil forming peptides for the induction of silver nanoparticles
Coiled-coil forming peptides for the induction of silver nanoparticles
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DOI：
10.1016/j.bbrc.2016.03.042
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发表时间：
2016-04-08
影响因子：
3.1
通讯作者：
Jerala, Roman
中科院分区：
文献类型：
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作者：
Abram, Sabina Bozic;Aupic, Jana;Jerala, Roman
具有明确序列模式的生物聚合物为银纳米颗粒（AgNP）的形成提供了一种有吸引力的替代方案。测试了一组卷曲螺旋二聚体形成肽的 AgNP 形成能力。其中 17 个肽在中性 pH 值的水溶液中介导 AgNP 的形成，而卷曲螺旋二聚体的形成则抑制纳米颗粒的生成。关于序列和功能之间关系的 QSAR 回归模型表明，在这种肽类型中，KXQQ 和 KXEE 模式是有利的，而 Ala 残基似乎具有抑制作用。获得的纳米颗粒的 UV-VIS 光谱在 420 nm 左右出现峰值，这是尺寸范围在 40 nm 左右的 AgNP 的典型峰值，动态光散射和透射电子显微镜证实了这一点。肽诱导的 AgNP 即使在 15 分钟的接触时间后也表现出良好的抗菌活性，而在相同浓度下它们对人体细胞的毒性较低。这些结果表明，我们设计的肽在温和条件下产生具有抗菌活性的AgNP，可用于抗菌涂层。 (C) 2016 Elsevier Inc. 保留所有权利。
Biopolymers with defined sequence patterns offer an attractive alternative for the formation of silver nanoparticle (AgNP). A set of coiled-coil dimer forming peptides was tested for their AgNP formation ability. Seventeen of those peptides mediated the formation of AgNPs in aqueous solution at neutral pH, while the formation of a coiled-coil dimer inhibited the nanoparticle generation. A QSAR regression model on the relationship between sequence and function suggests that in this peptide type the patterns KXQQ and KXEE are favorable, whereas Ala residues appear to have an inhibitory effect. UV-VIS spectra of the obtained nanoparticles gave a peak at around 420 nm, typical for AgNPs in the size range around 40 nm, which was confirmed by dynamic light scattering and transmission electron microscopy. Peptide induced AgNPs exhibited good antibacterial activity, even after a 15 min contact time, while they had low toxicity to human cells at the same concentrations. These results show that our designed peptides generate AgNPs with antibacterial activity at mild conditions and might be used for antibacterial coatings. (C) 2016 Elsevier Inc. All rights reserved.