Rapid In Vivo Quantification of Creatine Kinase Activity by Phosphorous-31 Magnetic Resonance Spectroscopic Fingerprinting (31P-MRSF).
Rapid In Vivo Quantification of Creatine Kinase Activity by Phosphorous-31 Magnetic Resonance Spectroscopic Fingerprinting (31P-MRSF).
复制标题
通过磷 31 磁共振光谱指纹图谱 (31P-MRSF) 快速体内定量肌酸激酶活性。
DOI：
10.1007/978-1-0716-1803-5_31
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yu,Xin
中科院分区：
文献类型：
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作者：
Wang,Charlie;Kim,Kihwan;Yu,Xin
肌酸激酶（CK）在组织代谢中起着重要作用，它在代谢紊乱时提供一种缓冲机制来维持三磷酸腺苷（ATP）的恒定供应。采用磁化转移技术的磷-31磁共振波谱（31 P-MRS）是测量肌酸激酶合成ATP速率的唯一无创方法。然而，由于低浓度的磷酸盐代谢产物，当前的31 P-MRS方法需要较长的采集时间来实现足够的测量精度。在本章中，我们提出了一个新的数据采集和参数估计框架，31 P磁共振光谱指纹（31 P-MRSF）方法，用于快速定量的CK反应速率常数在小实验室动物的后肢。
Creatine kinase (CK) plays an important role in tissue metabolism by providing a buffering mechanism for maintaining a constant supply of adenosine triphosphate (ATP) during metabolic perturbations. Phosphorous-31 magnetic resonance spectroscopy (31P-MRS) employing magnetization transfer techniques is the only noninvasive method for measuring the rate of ATP synthesis via creatine kinase. However, due to the low concentrations of phosphate metabolites, current31P-MRS methods require long acquisition time to achieve adequate measurement accuracy. In this chapter, we present a new framework of data acquisition and parameter estimation, the31P magnetic resonance spectroscopic fingerprinting (31P-MRSF) method, for rapid quantification of CK reaction rate constant in the hindlimb of small laboratory animals.