Functional imaging within individual pain fibres ex vivo with optical microscopy
Functional imaging within individual pain fibres ex vivo with optical microscopy
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DOI：
10.1016/j.jneumeth.2011.04.015
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发表时间：
2011-06-15
影响因子：
3
通讯作者：
Mulligan, Sean J.
中科院分区：
文献类型：
--
作者：
Baillie, Landon D.;Hagen, Volker;Mulligan, Sean J.
在这里，我们介绍了一个简单的实验方法来研究传入疼痛纤维的生理学。我们已经开发了一种用于光学显微荧光成像的小鼠整块硬脑膜颅骨制备，以直接研究选择性识别的个体伤害性纤维游离神经末梢的生理功能。使用宽场落射荧光显微镜的功能光学成像与电生理刺激，药理学操作和笼状化合物的UV光解相结合。对于第一次，我们显示高分辨率的功能成像的单一动作电位诱发的荧光瞬变，以及在个别伤害性纤维终端的子和阈上钙信号事件。这种新的实验方法为研究伤害性神经纤维的生理学和病理生理学开辟了一个新的窗口。(C)2011 Elsevier B.V.保留所有权利。
Here we introduce a simple experimental approach for studying afferent pain fibre physiology. We have developed a mouse en bloc dural-skull preparation for optical microfluorometric imaging to directly study the physiological functioning in selectively identified, individual nociceptive fibre free nerve endings. Functional optical imaging using widefield epifluorescence microscopy was combined with electrophysiological stimulations, pharmacological manipulations, and the UV photolysis of caged compounds. For the first time, we show high-resolution functional imaging of single action potential-evoked fluorescent transients, as well as sub- and supra-threshold calcium signaling events within individual nociceptive fibre terminations. This novel experimental approach opens up a new window for studying nociceptive fibre physiology and pathophysiology. (C) 2011 Elsevier B.V. All rights reserved.