Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice
Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice
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DOI：
10.1016/j.virol.2008.11.044
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发表时间：
2009-02-05
期刊：
影响因子：
3.7
通讯作者：
Chen, Hualan
中科院分区：
文献类型：
--
作者：
Fan, Shufang;Deng, Guohua;Chen, Hualan
A/duck/Guangxi/53/2002（DKGX/53）和A/duck/Fujian/01/2002（DKFJ/01）是H5 N1禽流感病毒，在鸡中是致命的。然而，在小鼠中，DKFJ/01是高致病性的，而DKGX/53显示低致病性。在这项研究中，我们使用反向遗传学证明，这两种病毒的M1蛋白的位置30和215的两个氨基酸残基是小鼠致病性的重要决定因素。因此，我们首次证明了M1蛋白有助于H5 N1病毒在小鼠中的毒力，并且显示出减弱毒力的氨基酸残基可以在流感病毒候选物中靶向用于活疫苗开发。(C)2008年爱思唯尔公司All rights reserved.
A/duck/Guangxi/53/2002 (DKGX/53) and A/duck/Fujian/01/2002 (DKFJ/01) are H5N1 avian influenza viruses that are lethal in chickens. In mice, however, DKFJ/01 is highly pathogenic, whereas DKGX/53 displays low pathogenicity. In this study, we used reverse genetics to demonstrate that two amino acid residues at positions 30 and 215 of the M1 protein of these two viruses are important determinants for pathogenicity in mice. We thus firstly prove the M1 protein contributes to the virulence of H5N1 viruses in mice, and the amino acid residues shown to attenuate the virulence could be targeted in influenza virus candidates for live vaccine development. (C) 2008 Elsevier Inc. All rights reserved.