High-performance liquid chromatographic determination of bergenin in different Bergenia species
High-performance liquid chromatographic determination of bergenin in different Bergenia species
复制标题
高效液相色谱法测定不同岩白菜属植物中岩白菜素的含量
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
A. Rawat
中科院分区：
文献类型：
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作者：
D. Singh;S. Srivastava;R. Govindarajan;A. Rawat
由于以往的化学研究表明岩白菜素和没食子酸是岩白菜素和没食子酸的重要生物活性成分，因此建立了一种简单、高精度的反相高效液相色谱与光电二极管阵列检测相结合的方法，用于岩白菜、纤毛岩白菜和岩白菜中这些化合物的同时测定。纤毛白菜和白花菜的岩白菜素含量最高，分别为3.275%和3.277%，白菜的岩白菜素含量为2.419%。
SUMMARY Because previous chemical investigations have revealed the presence of bergenin and gallic acid as important bioactive components of Bergenia, a simple, highly precise RP-HPLC method coupled with photodiode-array detection has been developed and validated for simultaneous determination of these compounds in the Bergenia species B. ligulata (Wall) Eng., B. ciliata (Royle) Raizada, and B. stracheyi Engl. B. ciliata and B. stracheyi were found to contain the most bergenin, 3.275 and 3.277%, respectively; B. ligulata contained 2.419% bergenin.