Effects of oligoDNA template length and sequence on binary self-assembly of a nucleotide bolaamphiphile.
Effects of oligoDNA template length and sequence on binary self-assembly of a nucleotide bolaamphiphile.
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DOI：
10.1039/b711687j
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发表时间：
2007-10
影响因子：
3.2
通讯作者：
Rika Iwaura;Y. Kikkawa;M. Ohnishi-Kameyama;T. Shimizu
中科院分区：
文献类型：
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作者：
Rika Iwaura;Y. Kikkawa;M. Ohnishi-Kameyama;T. Shimizu
核苷酸bola两亲物1（其中3 '-磷酸化胸苷部分连接到长寡亚甲基链的每一端）与10-、20-、30-或40-梅里克单链寡腺苷酸（2、3、4或5）的模板自组装导致在0.1x Tris/EDTA（TE）缓冲溶液中形成右旋螺旋纳米纤维。螺旋节距随着寡腺苷酸模板长度的增加而增加。由寡腺苷酸和寡胞苷酸序列（6、7和8）组成的DNA也作为模板诱导螺旋结构的形成。纳米纤维的直径保持恒定（6- 6.6nm），与所使用的模板无关。1与4的二元自组装也产生了更高阶的双链纳米纤维。
Templated self-assembly of nucleotide bolaamphiphile 1 (in which a 3'-phosphorylated thymidine moiety is connected to each end of a long oligomethylene chain) with a 10-, 20-, 30-, or 40-meric single-stranded oligoadenylic acid (2, 3, 4, or 5) led to the formation of right-handed helical nanofibers in 0.1x Tris/EDTA (TE) buffer solutions. The helical pitch increased as the length of the oligoadenylic acid template increased. DNA composed of oligoadenylic and oligocytidylic acid sequences (6, 7, and 8) also acted as templates to induce the formation of helical nanofiber structures. The diameter of the nanofibers remained constant (6-6.6 nm) irrespective of the template used. The binary self-assembly of 1 with 4 also produced higher-order, double-stranded nanofibers.