PREPARATION AND APPLICATION OF PROCION YELLOW STARCH FOR AMYLASE ASSAY
PREPARATION AND APPLICATION OF PROCION YELLOW STARCH FOR AMYLASE ASSAY
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DOI：
10.1016/0009-8981(80)90179-5
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发表时间：
1980-01-01
影响因子：
5
通讯作者：
JUNG, DH
中科院分区：
文献类型：
--
作者：
JUNG, DH
通过Procion Yellow染料与淀粉偶联，合成了一种用于测定淀粉酶活性的新型淀粉衍生物。该反应的产物呈深黄色，很容易悬浮在水中、中性缓冲溶液中，也可以悬浮在酸性溶液中。来自胰酶、唾液、尿液和血清的淀粉酶很容易水解这种显色底物。描述了一种基于使用 Procion 黄淀粉底物测定淀粉酶活性的方法。该过程需要 0.1 ml 样品和 30 分钟的孵育时间。在 420 nm 处测量酶水解释放的可溶性发色团，在此波长下淀粉酶活性-吸光度关系呈线性。该方法的正常范围是 50-150 Somogyi 单位/dl。
A new starch derivative for the determination of amylase activity was synthesized by coupling Procion Yellow dye with starch. The product of this reaction is intensely yellow and is easily suspended in water in neutral buffer solution, and also in acidic solution. Amylase from pancreatin, saliva, urine and serum readily hydrolyzes this chromogenic substrate. A method for determining amylase activity based on the use of the Procion Yellow Starch substrate is described. The procedure requires 0.1 ml of sample and an incubation time of 30 min. The soluble chromogen, which is liberated by enzymatic hydrolysis, is measured at 420 nm and the amylase activity-absorbance relationship at this wavelength is linear. The normal range for this method is 50-150 Somogyi units/dl.