UNILATERAL OLFACTORY DEPRIVATION - EFFECTS ON SUCCINATE-DEHYDROGENASE HISTOCHEMISTRY AND [H-3] LEUCINE INCORPORATION IN THE OLFACTORY MUCOSA
UNILATERAL OLFACTORY DEPRIVATION - EFFECTS ON SUCCINATE-DEHYDROGENASE HISTOCHEMISTRY AND [H-3] LEUCINE INCORPORATION IN THE OLFACTORY MUCOSA
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DOI：
10.1016/0165-3806(91)90171-e
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发表时间：
1991-10-21
期刊：
影响因子：
--
通讯作者：
STEWART, JS
中科院分区：
文献类型：
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作者：
BRUNJES, PC;CAGGIANO, AO;STEWART, JS
手术关闭一个外鼻孔减少了通过鼻腔一半的气流，减少了气味进入受体片。在大鼠中，单侧鼻孔闭塞在出生时进行的结果在嗅球的大小，主要的中央中继，30天后检查时，大幅度减少。先前的研究表明，在鼻孔关闭后数小时内，[H-3]2-脱氧葡萄糖（2-DG）和[H-3]亮氨酸在球部的摄取迅速减少。本研究探讨是否可以观察到类似的快速变化，在感觉周边。在P1闭塞并在P3用琥珀酸脱氢酶组织化学检查的幼仔显示鼻腔闭合侧染色减少，表明闭塞导致粘膜代谢减少。在P6检查的幼仔中观察到较大的染色差异。[H-3]直到闭塞后30天，鼻中隔两侧的亮氨酸掺入非常相似，表明蛋白质合成的变化不太显著。结果表明，鼻孔关闭确实对粘膜功能有快速影响，但表明这些变化与在球部观察到的变化不同。
Surgically closing one external naris reduces airflow through one half of the nasal cavity, decreasing the access of odors to the receptor sheet. In rats, unilateral naris occlusion performed near birth results in large reductions in the size of the olfactory bulb, the primary central relay, when examined 30 days later. Previous research has demonstrated that there is a rapid reduction in [H-3]2-deoxyglucose (2-DG) and [H-3]leucine uptake in the bulb within hours after naris closure. The present study examined whether similar rapid changes could be observed in the sensory periphery. Pups occluded on P1 and examined on P3 with succinate dehydrogenase histochemistry exhibited reduced staining on the closed side of the nasal cavity, suggesting occlusion results in reductions in mucosal metabolism. Larger differences in staining were observed in pups examined at P6. [H-3]Leucine incorporation was quite similar on both sides of the nasal septum as late as 30 days post occlusion, suggesting less dramatic changes in protein synthesis. The results suggest that naris closure does indeed have rapid effects on mucosal function, but indicate that the changes are different than those observed in the bulb.