Preservation of large yellow croaker (Pseudosciaena crocea) by Coagulin L1208, a novel bacteriocin produced by Bacillus coagulans L1208
Preservation of large yellow croaker (Pseudosciaena crocea) by Coagulin L1208, a novel bacteriocin produced by Bacillus coagulans L1208
复制标题
利用凝结芽孢杆菌 L1208 产生的新型细菌素 Coagulin L1208 保鲜大黄鱼（Pseudosciaena crocea）
DOI：
10.1016/j.ijfoodmicro.2017.11.012
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发表时间：
2018-02-02
影响因子：
5.4
通讯作者：
Wang, Yanbo
中科院分区：
文献类型：
--
作者：
Fu, Linglin;Wang, Chong;Wang, Yanbo
大黄鱼（Pseudosciaena crocea）是一种重要的经济养殖鱼类，具有丰富的营养价值。然而，由于其高蛋白质和水分含量，它很容易分解，导致相当大的经济损失和对消费者健康的不利影响。在这里，我们评估了细菌菌株凝结芽孢杆菌L1208（Bcoa）在4 ℃贮藏期间保存大黄鱼的功能，发现Bcoa显著延长了货架期。进一步的研究表明，Bcoa主要通过抑制腐败菌的生长来延长贮藏时间。此外，一个新的细菌素，命名为凝固蛋白L1208和Bcoa产生的，纯化和鉴定的N-末端测序。最后，对凝固蛋白L1208在大黄鱼保鲜过程中抑制腐败菌的活性进行了评价。本研究揭示了Bcoa对大黄鱼保鲜的作用机制，并确定了凝固蛋白L1208作为一种潜在的新型防腐剂。
Large yellow croaker (Pseudosciaena crocea) is a cultivated fish of great economic importance and abundant nutritional value. However, due to its high protein and water contents, it is susceptible to decomposition, leading to considerable economic loss and adverse effects on consumer health. Here, we assessed the function of the bacterial strain Bacillus coagulans L1208 (Bcoa) in preserving large yellow croaker during storage at 4 degrees C and found that Bcoa elongates the shelf-life significantly. Further investigations showed that Bcoa prolongs the storage time mainly by suppressing the growth of spoilage bacteria. Moreover, a novel bacteriocin, designated as Coagulin L1208 and produced by Bcoa, was purified and identified by N-terminal sequencing. Finally, the activity of Coagulin L1208 for suppressing spoilage bacteria during the preservation of large yellow croaker was assessed. Our results reveal the mechanism by which Bcoa aids the preservation of large yellow croaker and identify Coagulin L1208 as a potential novel antiseptic.