Quantitative assessment of partial vascular occlusions in a swine pedicle flap model using spatial frequency domain imaging.
Quantitative assessment of partial vascular occlusions in a swine pedicle flap model using spatial frequency domain imaging.
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DOI：
10.1364/boe.4.000298
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发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Durkin AJ
中科院分区：
文献类型：
--
作者：
Ponticorvo A;Taydas E;Mazhar A;Scholz T;Kim HS;Rimler J;Evans GR;Cuccia DJ;Durkin AJ
组织转移皮瓣的使用已成为重建或替换受损组织的常见且有效的技术。虽然与这些手术相关的总体失败率相对较低（5-10%），但需要额外手术的组织瓣的失败率明显较高（40-60%）。其原因主要是由于缺乏客观评估手术后组织健康的技术。在这里，我们研究了空间频域成像（SFDI）作为一种潜在的工具来做到这一点。通过将多个波长的宽场图案化照明投射到组织表面上，SFDI能够在大视场内量化氧合血红蛋白和脱氧血红蛋白的绝对浓度。我们已经通过使用受控血管闭塞系统在猪蒂皮瓣模型中评估了SFDI的灵敏度，该系统将静脉或动脉中的血流量减少了25%、50%、75%或100%的基线值。SFDI能够检测至少50%的部分动脉闭塞和至少25%的部分静脉闭塞中的氧合血红蛋白、脱氧血红蛋白或组织氧饱和度的显著变化。这表明SFDI足够敏感，可以量化部分闭塞期间组织血红蛋白状态的变化，因此有可能成为早期预测组织瓣失败的有力工具。
The use of tissue transfer flaps has become a common and effective technique for reconstructing or replacing damaged tissue. While the overall failure rate associated with these procedures is relatively low (5-10%), the failure rate of tissue flaps that require additional surgery is significantly higher (40-60%). The reason for this is largely due to the absence of a technique for objectively assessing tissue health after surgery. Here we have investigated spatial frequency domain imaging (SFDI) as a potential tool to do this. By projecting wide-field patterned illumination at multiple wavelengths onto a tissue surface, SFDI is able to quantify absolute concentrations of oxygenated and deoxygenated hemoglobin over a large field of view. We have assessed the sensitivity of SFDI in a swine pedicle flap model by using a controlled vascular occlusion system that reduced blood flow by 25%, 50%, 75%, or 100% of the baseline values in either the vein or artery. SFDI was able to detect significant changes for oxygenated hemoglobin, deoxygenated hemoglobin, or tissue oxygen saturation in partial arterial occlusions of at least 50% and partial venous occlusions of at least 25%. This shows SFDI is sensitive enough to quantify changes in the tissue hemoglobin state during partial occlusions and thus has the potential to be a powerful tool for the early prediction of tissue flap failure.