Genetic recombination in disgust-associated bitter taste-responsive neurons of the central nucleus of amygdala in male mice
Genetic recombination in disgust-associated bitter taste-responsive neurons of the central nucleus of amygdala in male mice
复制标题
雄性小鼠杏仁核中央核厌恶相关苦味反应神经元的基因重组
DOI：
10.1016/j.neulet.2020.135456
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发表时间：
2021
影响因子：
2.5
通讯作者：
Tanabe Tsutomu
中科院分区：
文献类型：
--
作者：
Tanaka Daisuke H.;Li Shusheng;Mukae Shiori;Tanabe Tsutomu
苦味物质会引起特定的口面和躯体行为反应，如小鼠、猴子和人类的张口反应。这些反应被认为代表了情感厌恶，因此，理解这些反应的神经基础将为理解情感厌恶铺平道路。关键是要识别和访问特定的神经元集合，这些神经元集合被苦味物质激活，如奎宁，摄入这些物质会引起厌恶反应。然而，进入奎宁激活的神经元的方法尚未完全建立。在这里，我们显示的证据表明，有针对性的重组在活跃的人群（TRAP）的方法，诱导遗传重组的奎宁激活的杏仁核（CeA）的中央核神经元。CeA是众所周知的大脑情绪中心之一。我们发现，口内注射奎宁引起的恶心反应使CeA中cFos阳性细胞和Arc阳性细胞均增加。利用Arc-CreER; Ai 3 TRAP小鼠，我们在奎宁激活的神经元中诱导基因重组，并用荧光蛋白标记它们。我们证实奎宁TRAPed荧光标记细胞优先共表达ARC奎宁输液后。我们的研究结果表明，TRAP方法可用于访问特定的功能神经元在CeA。
A bitter substance induces specific orofacial and somatic behavioral reactions such as gapes in mice as well as monkeys and humans. These reactions have been proposed to represent affective disgust, and therefore, understanding the neuronal basis of the reactions would pave the way to understand affective disgust. It is crucial to identify and access the specific neuronal ensembles that are activated by bitter substances, such as quinine, the intake of which induces disgust reactions. However, the method to access the quinine-activated neurons has not been fully established yet. Here, we show evidence that a targeted recombination in active populations (TRAP) method, induces genetic recombination in the quinine-activated neurons in the central nucleus of the amygdala (CeA). CeA is one of the well-known emotional centers of the brain. We found that the intraoral quinine infusion, that resulted in disgust reactions, increased bothcFos-positive cells andArc-positive cells in the CeA. By usingArc-CreER;Ai3 TRAP mice, we induced genetic recombination in the quinine-activated neurons and labelled them with fluorescent protein. We confirmed that the quinine-TRAPed fluorescently-labelled cells preferentially coexpressedArcafter quinine infusion. Our results suggest that the TRAP method can be used to access specific functional neurons in the CeA.