Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China
Molecular characterization of a Class I Newcastle disease virus strain isolated from a pigeon in China
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DOI：
10.1080/03079457.2016.1153036
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发表时间：
2016-01-01
期刊：
影响因子：
2.8
通讯作者：
Yang, Zengqi
中科院分区：
文献类型：
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作者：
Ren, Shanhui;Xie, Xiumei;Yang, Zengqi
为了阐明自然宿主在纽卡斯尔病病毒（NDV）生态学中的作用，进行了持续监测。本研究对从一只无症状鸽中分离到的NDV毒株进行了序列测定和分析。结果表明，该分离物基因组全长为15,198个核苷酸，基因序列为3 '-NP-P-M-F-HN-L-5'。该分离株为弱毒型，脑内致病指数为0.00，平均死亡时间大于148 h。该分离物在F蛋白切割位点具有-E-112-R-Q-E-R-L-117-基序。此外，在融合蛋白（F）和血凝素-神经氨酸酶蛋白（HN）蛋白的功能结构域中分别鉴定出7个和13个氨基酸取代。F和HN蛋白的中和表位的氨基酸分析显示，在分离物中分别有3个和10个氨基酸取代。系统发育分析将该分离物归类为Ⅰ类Ib基因型。该分离株与2005 - 2013年我国南方和东部地区野鸟和水禽中分离到的NDV毒株具有较高的同源性。据我们所知，本研究是第一次报告从鸽中分离到属于I类Ib基因型的NDV毒株，而不是传统的II类VI基因型或其他亚基因型。本研究为阐明I类病毒的分布和进化提供了信息，为进一步预防NDV提供了依据。
Constant monitoring is performed to elucidate the role of natural hosts in the ecology of Newcastle disease virus (NDV). In this study, an NDV strain isolated from an asymptomatic pigeon was sequenced and analysed. Results showed that the full-length genomes of this isolate were 15,198 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. This NDV isolate was lentogenic, with an intracerebral pathogenicity index of 0.00 and a mean time of death more than 148 h. The isolate possessed a motif of -E-112-R-Q-E-R-L-117- at the F protein cleavage site. In addition, 7 and 13 amino acid substitutions were identified in the functional domains of fusion protein (F) and haemagglutinin-neuraminidase protein (HN) proteins, respectively. Analysis of the amino acids of neutralizing epitopes of F and HN proteins showed 3 and 10 amino acid substitutions, respectively, in the isolate. Phylogenetic analysis classified the isolate into genotype Ib in Class I. This isolate shared high homologies with the NDV strains isolated from wild birds and waterfowl in southern and eastern parts of China from 2005 to 2013. To our knowledge, this study is the first to report a NDV strain isolated from pigeon that belongs to genotype Ib in Class I, rather than to the traditional genotype VI or other sub-genotypes in Class II. This study provides information to elucidate the distribution and evolution of Class I viruses for further NDV prevention.