Karyotyping mouse chromosomes by multiplex-FISH (M-FISH)
Karyotyping mouse chromosomes by multiplex-FISH (M-FISH)
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DOI：
10.1023/a:1016696303479
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发表时间：
2001-04-01
影响因子：
2.6
通讯作者：
Speicher, MR
中科院分区：
文献类型：
--
作者：
Jentsch, I;Adler, ID;Speicher, MR
小鼠染色体的核型分析是一门技巧性的艺术，即使对于经验丰富的细胞遗传学家来说，这也是一项费力的工作。随着人类疾病小鼠模型数量的不断增加，对自动化小鼠核型分析系统的需求也不断增加。这里展示了一种基于多重荧光原位杂交（M-FISH）技术的小鼠染色体核型分析系统。该系统在许多具有数值和结构畸变的小鼠个体上进行了测试，并验证了其再现性和稳健性。小鼠 M-FISH 应该是分析小鼠染色体重排的有价值的工具。
Karyotyping of mouse chromosomes is a skillful art, which is laborious work even for experienced cytogeneticists. With the growing number of mouse models for human diseases, there is an increasing demand for automated mouse karyotyping systems. Here, such a karyotyping system for mouse chromosomes based on the multiplex-fluorescence in-situ hybridization (M-FISH) technology is shown. The system was tested on a number of individual mice with numerical and structural aberrations and its reproducibility and robustness verified. Mouse M-FISH should be a valuable tool for the analysis of chromosomal rearrangements in mice.