Heterologous HA DNA vaccine prime-inactivated influenza vaccine boost is more effective than using DNA or inactivated vaccine alone in eliciting antibody responses against H1 or H3 serotype influenza viruses
Heterologous HA DNA vaccine prime-inactivated influenza vaccine boost is more effective than using DNA or inactivated vaccine alone in eliciting antibody responses against H1 or H3 serotype influenza viruses
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DOI：
10.1016/j.vaccine.2008.04.073
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发表时间：
2008-07-04
期刊：
影响因子：
5.5
通讯作者：
Lu, Shan
中科院分区：
文献类型：
--
作者：
Wang, Shixia;Parker, Chris;Lu, Shan
三价灭活疫苗(TIV)用于预防人类感染季节性流感病毒，但该疫苗的免疫原性可能受到以往流感疫苗的免疫效果或接触抗原性相关流感病毒的影响。目前的研究检验了一种临床许可的TIV在对流感抗原幼稚的兔身上的免疫原性。动物接种许可的TIV、双价(H1和H3)HA DNA疫苗或两者的组合。采用酶联免疫吸附试验(EL ISA)检测血清抗流感病毒免疫球蛋白抗体的时间和峰值水平。通过血凝抑制和微量中和法测定对A/NewCaledony/20/99(H1N1)或A/巴拿马/2007/99(H3N2)流感病毒的功能性抗体反应。我们的结果表明，在血清阴性动物中，TIV的免疫原性较低。更重要的是，异源DNA Prime-TIV Boost方案比单独使用TIV或DNA疫苗的同源Prime-Boost方案更具免疫原性。这一发现证明了进一步研究HA DNA疫苗作为下一代针对季节性或大流行性流感病毒感染的疫苗的启动免疫原的合理性。(C)2008爱思唯尔有限公司。保留所有权利。
The trivalent inactivated vaccine (TIV) is used to prevent seasonal influenza virus infection in humans, however, the immunogenicity of this vaccine may be influenced by the priming effect of previous influenza vaccinations or exposure to antigenically related influenza viruses. The current study examines the immunogenicity of a clinically licensed TIV in rabbits naive to influenza antigens. Animals were immunized with either the licensed TIV, a bivalent (H1 and H3) HA DNA vaccine or the combination of both. Temporal and peak level serum anti-influenza virus IgG responses were determined by enzyme-linked immunosorbent assay (ELISA). Functional antibody responses were measured by hemagglutination inhibition and microneutralization against either A/NewCaledonia//20/99 (H1N1) or A/Panama/2007/99 (H3N2) influenza viruses. Our results demonstrate that the immunogenicity of the TIV is low in sero-negative animals. More significantly, the heterologous DNA prime-TIV boost regimen was more immunogenic than the homologous prime-boost using either TIV or DNA vaccines alone. This finding justifies further investigation of HA DNA vaccines as a priming immunogen for the next generation of vaccines against seasonal or pandemic influenza virus infections. (C) 2008 Elsevier Ltd. All rights reserved.