Distribution of virulence-associated genes and antimicrobial susceptibility in clinical Acinetobacter baumannii isolates.
Distribution of virulence-associated genes and antimicrobial susceptibility in clinical Acinetobacter baumannii isolates.
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DOI：
10.18632/oncotarget.24651
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发表时间：
2018-04-24
期刊：
影响因子：
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通讯作者：
Jia X
中科院分区：
文献类型：
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作者：
Liu C;Chang Y;Xu Y;Luo Y;Wu L;Mei Z;Li S;Wang R;Jia X
鲍曼不动杆菌无疑是临床上最重要的病原体之一。A.鲍曼不动杆菌是医院获得性医院感染的原因。与大量的耐药流行病学研究不同，鲍曼尼，毒力分子流行病学研究较少。在这里，我们收集了88个A。目的：研究鲍曼不动杆菌临床分离株对10种常用抗生素的敏感性，并分析9种耐药相关基因的分布，探讨鲍曼不动杆菌多药耐药（MDR）和非MDR临床分离株耐药相关基因的基本特征。鲍曼不动杆菌。MIC结果表明，环丙沙星的耐药率（68.2%，60/88），庆大霉素（67.0%，59/88），阿米卡星（58.0%，51/88），妥布霉素（58.0%，51/88），强力霉素（67.0%，59/88），美罗培南（54.5%，48/88）和亚胺培南除左氧氟沙星（34.1%，30/88）、米诺环素（1.1%，1/88）和多粘菌素B（0%，0/88）外，其余均在50%以上。脉冲场凝胶电泳（PFGE）分析表明，MDRA耐药率为100%~ 100%;鲍曼不动杆菌在流行组中占优势（79.5%，44/58），而在散发组中仅占6.7%（2/30）。毒力基因的分布表明，除traT（0%）外，其它毒力基因bap（95.5%）、surA 1（92.0%）、BasD（92.0%）、paaE（88.6%）、pld（87.5%）、BauA（62.5%）、omp 33 -36（59.1%）和pglC（53.4%）所占比例较高。总体而言，我们的研究结果表明，MDR分离株在流行A中占主导地位。鲍曼不动杆菌分离株，并含有很高比例的毒力基因，这可能导致高风险，高致病性和高治疗挑战。
Acinetobacter baumannii is undoubtedly one of the most clinically significant pathogens. The multidrug resistance and virulence potential of A. baumannii are responsible for hospital-acquired nosocomial infections. Unlike numerous investigations on the drug-resistant epidemiology of A. baumanni, virulence molecular epidemiology is less studied. Here, we collected 88 A. baumannii clinical isolates, tested their antimicrobial susceptibility to 10 commonly used antibiotics and analyzed the distribution of 9 selected virulence-associated genes, aims to investigate the primary characteristics of the virulence-associated genes that exist in clinically multidrug resistant (MDR) and non-MDR isolates of A. baumannii. The MIC results showed the resistance rates of ciprofloxacin (68.2%, 60/88), gentamicin (67.0%, 59/88), amikacin (58.0%, 51/88), tobramycin (58.0%, 51/88), doxycycline (67.0%, 59/88), meropenem (54.5%, 48/88) and imipenem (65.9%, 58/88) were all above 50%, except for levofloxacin (34.1%, 30/88), minocycline (1.1%, 1/88) and polymyxin B (0%, 0/88). The Pulsed field gel electrophoresis (PFGE) analysis revealed that the resistance rate of MDR A. baumannii isolates in the Epidemic group was predominant (79.5%, 44/58), but in the Sporadic group was only 6.7% (2/30). Further investigation on the distribution of virulence genes showed the virulence genes bap (95.5%), surA1 (92.0%), BasD (92.0%), paaE (88.6%), pld (87.5%), BauA (62.5%), omp33-36 (59.1%) and pglC (53.4%) were accounted for high proportion, except for traT (0%). Overall, our results revealed that MDR isolates predominated in the Epidemic A. baumannii isolates, and contained a very high proportion of virulence genes, which may lead to high risk, high pathogenicity and high treatment challenge.