MOLECULAR CHARACTERIZATION AND PROPOSAL OF A NEOTYPE STRAIN FOR BARTONELLA-BACILLIFORMIS
MOLECULAR CHARACTERIZATION AND PROPOSAL OF A NEOTYPE STRAIN FOR BARTONELLA-BACILLIFORMIS
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DOI：
10.1128/jcm.29.7.1299-1302.1991
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发表时间：
1991-07-01
影响因子：
9.4
通讯作者：
STEIGERWALT, AG
中科院分区：
文献类型：
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作者：
BRENNER, DJ;OCONNOR, SP;STEIGERWALT, AG
巴尔通氏杆菌是巴尔通体病的病原体，通过DNA杂交、鸟嘌呤+胞嘧啶含量、基因组大小和16S rRNA测序对其进行生化特征分析。我们收集的两个菌株的DNA在55℃(最佳重新结合标准)下进行的羟基磷灰石反应中显示出97%的相关性，在70℃(严格的重新结合标准)下进行的反应中显示出100%的相关性。没有证据表明相关序列之间存在分歧。在65摄氏度的杂交反应中，杆状杆菌DNA与猫抓病杆菌或与猫抓病杆菌属于同一属的第二个物种的菌株没有相关性。两株杆状芽孢杆菌DNA中鸟嘌呤+胞嘧啶的含量分别为39%和40%。通过分光光度法测定一半变性DNA形成双链所需的时间进行的时程再结合，表明杆状芽孢杆菌的基因组大小约为4×10(8)。16S rRNA序列分析表明，杆状芽孢杆菌属于变形杆菌纲紫色细菌的α-2亚群，其近亲是Rochalimaea quintana和布鲁氏菌bortus。菌株KC583(=Herer020/F12，63=atcc 35685)为杆状芽孢杆菌的模式菌株。
Bartonella bacilliformis, the etiologic agent of bartonellosis, was characterized biochemically and by DNA hybridization, guanine-plus-cytosine content, genome size, and 16S rRNA sequencing. DNAs from the two strains in our collection exhibited 97% relatedness in hydroxyapatite reactions done at 55-degrees-C (optimal reassociation criterion) and 100% relatedness in reactions done at 70-degrees-C (stringent reassociation criterion). There was no evidence of divergence within the related sequences. B. bacilliformis DNA showed no relatedness to the cat scratch disease bacillus or to a strain of a second species in the same genus as the cat scratch disease bacillus in hybridization reactions done at 65-degrees-C. The guanine-plus-cytosine contents of DNAs from the two B. bacilliformis strains were 39 and 40 mol%. Time course reassociation, done by determining spectrophotometrically the time required for one-half of the denatured DNA to form duplexes, indicated that B. bacilliformis has a genome size of approximately 4 x 10(8). The 16S rRNA sequence analysis indicated that B. bacilliformis is in the alpha-2 subgroup of the purple bacteria, class Proteobacteria, and that its closest relatives are Rochalimaea quintana and Brucella abortus. Strain KC583 (= Herrer 020/F12,63 = ATCC 35685) is proposed as the type strain of B. bacilliformis.