ROLE OF CARDIOLIPIN IN THE FUNCTIONING OF MITOCHONDRIAL L-GLYCEROL-3-PHOSPHATE DEHYDROGENASE
ROLE OF CARDIOLIPIN IN THE FUNCTIONING OF MITOCHONDRIAL L-GLYCEROL-3-PHOSPHATE DEHYDROGENASE
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DOI：
10.1016/0006-291x(89)92414-5
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发表时间：
1989-02-28
影响因子：
3.1
通讯作者：
JANCSIK, V
中科院分区：
文献类型：
--
作者：
BELEZNAI, Z;JANCSIK, V
采用阿霉素原位诱导离体甲状腺功能亢进大鼠肝线粒体，研究心磷脂在FAD-linked l-甘油-3-磷酸脱氢酶功能中的作用。阿霉素对酶催化反应的表观动力学参数有影响。阿霉素的作用依赖于电子受体，这表明疏水受体和亲水受体存在不同的结合位点。假设阿霉素与线粒体结合时观察到的两个高原与药物穿透内膜的两个小叶之间存在相关性[Cheneval et al. (1985) J. Biol.]。化学，260,13003-13007]，我们可以推断两个小叶中的心磷脂主要影响电子受体结合位点。
Adriamycin was used in situ, in isolated liver mitochondria of hyperthyroid rats to study the role of cardiolipin in the functioning of FAD-linked L-glycerol-3-phosphate dehydrogenase. The apparent kinetic parameters of the reaction catalyzed by the enzyme were affected by adriamycin. The effect of adriamycin was dependent on the electron acceptor, suggesting the existence of distinct binding sites for hydrophobic and hydrophilic acceptors. Assuming a correlation between the two plateaus observed upon binding of adriamycin to the mitochondria and the penetration of the drug into the two leaflets of the inner membrane [Cheneval et al. (1985) J. Biol. Chem. 260, 13003-13007], we can deduce that cardiolipin in both leaflets influences predominantly the electron acceptor binding site(s).