Static magnetic field regulates Arabidopsis root growth via auxin signaling
Static magnetic field regulates Arabidopsis root growth via auxin signaling
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DOI：
10.1038/s41598-019-50970-y
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发表时间：
2019-10-07
影响因子：
4.6
通讯作者：
Huang, Jirong
中科院分区：
文献类型：
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作者：
Jin, Yue;Guo, Wei;Huang, Jirong
静磁场在许多生物体的生物过程中起着重要的作用。然而，在植物中，SMF的生物学意义和SMF作用的分子机制仍然在很大程度上未知。为了解决这些问题，我们用不同的SMF强度和方向处理拟南芥幼苗。从北极到南极的磁场方向被调整为与重力矢量平行（N 0）、相反（N180）和垂直。结果表明，除N180外，600 mT处理对根系生长有显著的促进作用，而300 mT处理对根系生长无显著影响。N 0处理导致分生组织的细胞分裂更活跃，并且通过在根尖中协调表达PIN 3和AUX 1来调节生长素含量。因此，在pin 3和aux 1突变体中，N 0促进的根生长消失。转录组学和基因本体分析表明，在根中85%的总基因显着下调N 0相比，未处理的质体生物过程中，如代谢和叶绿体发育丰富。最后，没有差异的根长之间观察到N 0-处理和未处理的双隐花色素突变体cry 1 cry 2的根。综上所述，我们的数据表明，SMF调节的根生长是由CRY和生长素信号通路介导的拟南芥。
Static magnetic field (SMF) plays important roles in biological processes of many living organisms. In plants, however, biological significance of SMF and molecular mechanisms underlying SMF action remain largely unknown. To address these questions, we treated Arabidopsis young seedlings with different SMF intensities and directions. Magnetic direction from the north to south pole was adjusted in parallel (N0) with, opposite (N180) and perpendicular to the gravity vector. We discovered that root growth is significantly inhanced by 600 mT treatments except for N180, but not by any 300 mT treatments. N0 treatments lead to more active cell division of the meristem, and higher auxin content that is regulated by coordinated expression of PIN3 and AUX1 in root tips. Consistently, N0-promoted root growth disappears in pin3 and aux1 mutants. Transcriptomic and gene ontology analyses revealed that in roots 85% of the total genes significantly down-regulated by N0 compared to untreatment are enriched in plastid biological processes, such as metabolism and chloroplast development. Lastly, no difference in root length is observed between N0-treated and untreated roots of the double cryptochrome mutant cry1 cry2. Taken together, our data suggest that SMF-regulated root growth is mediated by CRY and auxin signaling pathways in Arabidopsis.