Novel electrophoresis mechanism based on synchronous alternating drag perturbation.
Novel electrophoresis mechanism based on synchronous alternating drag perturbation.
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基于同步交替阻力扰动的新型电泳机制。
DOI：
10.1002/elps.200406140
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Whitehead,LorneA
中科院分区：
文献类型：
--
作者：
Marziali,Andre;Pel,Joel;Bizzotto,Dan;Whitehead,LorneA
我们提出了一种新的方法来传输溶液中的分子通过施加一个零时间平均交变动力的分子，并扰动的分子阻力系数与所施加的力同步，从而导致净漂移的方向所确定的相位的交变阻力扰动相对于交变力。我们应用电泳形式的方法来运输和浓缩凝胶中的DNA，使得所有分子平均远离最近的电极并朝向中心区域迁移。由于电极不占据该中心区域，因此该方法提供了在没有电极和相关电化学的区域中运输和聚焦DNA和其他带电分子的可能性。
We present a novel means of transporting molecules in solution by applying a zero‐time‐average alternating motive force to the molecules, and perturbing the molecular drag coefficient synchronously with the applied force, thus causing a net drift in a direction determined by the phase of the alternating drag perturbation relative to the alternating force. We apply an electrophoretic form of the method to transport and concentrate DNA in a gel, such that all molecules migrate on average away from the nearest electrode and toward a central region. Since an electrode does not occupy this central region, this method presents the possibility of transporting and focusing DNA and other charged molecules in regions free from electrodes and the associated electrochemistry.