CENTRAL PROLACTIN INFUSIONS STIMULATE MATERNAL-BEHAVIOR IN STEROID-TREATED, NULLIPAROUS FEMALE RATS
CENTRAL PROLACTIN INFUSIONS STIMULATE MATERNAL-BEHAVIOR IN STEROID-TREATED, NULLIPAROUS FEMALE RATS
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DOI：
10.1073/pnas.87.20.8003
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
LUPINI, CE
中科院分区：
文献类型：
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作者：
BRIDGES, RS;NUMAN, M;LUPINI, CE
进行了一系列实验，以确定中枢催乳素(PRL)是否以及在什么条件下会刺激雌性大鼠母体行为的发生，并确定PRL作用的可能神经部位。在摘除卵巢的实验中，用溴隐亭抑制内源性PRL水平的未分娩大鼠被测试母亲对寄养幼鼠的行为。在实验1、2和4中，女性也暴露在孕酮水平(第1-11天)和雌二醇(第11-17天)中。在实验1中，将四种剂量的绵羊催乳素(400 ng、2微克、10微克或50微克，但不是80毫克)注入侧脑室(第11-13天)，导致母性行为的迅速开始(第12-17天的行为测试)。这些剂量的PRL的刺激作用似乎是中心的，因为皮下注射50微克的绵羊PRL未能影响母体的反应性(实验2)。实验3表明，侧脑室注射催乳素的兴奋作用是类固醇依赖性的。输注10微克的绵羊催乳素或10微克的大鼠催乳素都不能在非类固醇治疗的动物中诱导母性行为。在最后的实验(第4号)中，将40 ng的绵羊催乳素注入类固醇治疗的大鼠的内侧视前区，导致明显的母性行为刺激。这些发现证明了PRL在刺激母体反应性方面的中心作用部位，并指出内侧视前区是PRL调节母体行为的关键神经部位。
A series of experiments were conducted to determine whether and under what conditions central prolactin (PRL) administration would stimulate the onset of maternal behavior in female rats and to identify possible neural sites of PRL action. In experiment ovariectomized, nulliparous rats whose endogenous PRL levels were suppressed with bromocriptine were tested for maternal behavior toward foster young. In experiments 1, 2, and 4, females were also exposed to pregnancy-like levels of progesterone (days 1-11) followed by estradiol (days 11-17). In experiment 1 infusions (days 11-13) of four doses of ovine PRL (400 ng, 2 .mu.g, 10 .mu.g, or 50 .mu.g, but not 80 ng) into the lateral ventricle resulted in a rapid onset of maternal behavior (behavioral testing, days 12-17). The stimulatory action of these doses of PRL appears to be central, since subcutaneous injections of 50 .mu.g of ovine PRL failed to affect maternal responsiveness (experiment 2). Experiment 3 indicated that the stimulatory effect of intracerebroventricularly administered PRL is steroid dependent. Infusions of either 10 .mu.g of ovine PRL or 10 .mu.g of rat PRL failed to induce maternal behavior in nonsteroid-treated animals. In the final experiment (no.4) bilateral infusions of 40 ng of ovine PRL into the medial preoptic area of steroid-treated rats resulted in a pronounced stimulation of maternal behavior. These findings demonstrate a central site of PRL action in the stimulation of maternal responsiveness and point to the medial preoptic area as a key neural site for PRL regulation of maternal behavior.