Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells
Effects of compressive stress on the expression of M-CSF, IL-1β, RANKL and OPG mRNA in periodontal ligament cells
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压缩应力对牙周膜细胞M-CSF、IL-1β、RANKL、OPG mRNA表达的影响
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Yoon
中科院分区：
文献类型：
--
作者：
Ji;K. Lee;J. Nahm;Yoon
目的：研究压力对人PDL细胞破骨细胞基因表达的影响，探讨人PDL细胞是否能产生破骨细胞基因。方法：从用于正畸治疗的饼干中提取人PDL细胞。通过增加盖玻璃的数量来调整压缩力。分别以0.5g/c、1.0g/c、2.0g/c、3.0g/c和4.0g/c㎡的压力作用0.5h、1.5h、6h、24h和48h。逆转录-聚合酶链式反应检测M-CSF、IL-1β、RANKL、OPG mRNA表达水平。结果：人PDL细胞可产生M-CSF mRNA。人PDL细胞在压力作用下，M-csf、IL-1β和RANKLmRNAs的表达呈强力性(高达2g/c㎡)和时间依赖性增加。然而，无论应激程度和持续时间如何，OPG mRNA的表达都是恒定的。结论：持续压力可诱导破骨细胞因子M-CSF、RANKL、IL-1β的表达。这些结果表明，压力诱导的体内破骨细胞形成部分受控于PDL细胞中M-CSF、RANKL和IL-1β的表达。(朝鲜矫形外科杂志2009；39(4)：248-256)
Objective: The aim of this study was to determine if human PDL cells can produce osteoclastogenic mRNA and examine how compressive stress affects the expression of osteoclastogenic mRNA in human PDL cells. Methods: Human PDL cells were obtained from biscupids extracted for orthodontic treatment. The compressive force was adjusted by increasing the number of cover glasses. PDL cells were subjected to a compressive force of 0.5, 1.0, 2.0, 3.0 or 4.0 g/c㎡ for 0.5, 1.5, 6, 24 or 48 hours. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed to examine levels of M-CSF, IL-1β, RANKL, OPG mRNA expression. Results: Human PDL cells could produce M-CSF mRNA. Human PDL cells under compressive stress showed increased M-CSF, IL-1β and RANKL mRNAs expression in a force (up to 2 g/c㎡) and time-dependent manner. However, OPG mRNA expression was constant regardless of the level and duration of stress. Conclusions: Continuous compressive stress induced the mRNA expression of osteoclastogenic cytokines including M-CSF, RANKL, IL-1β in PDL cells. Together with an unchanged OPG mRNA level, these results suggest that compressive stress-induced osteoclastogenesis in vivo is partly controlled by M-CSF, RANKL and IL-1β expression in PDL cells. (Korean J Orthod 2009;39(4):248-256)
影响因子：
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作者：
Roberts We;Goodwin Wc;Heiner
通讯作者：
Roberts We;Goodwin Wc;Heiner
巨噬细胞集落刺激因子的局部浓度介导破骨细胞分化。
DOI：
10.1152/ajpendo.1995.269.6.e1024
发表时间：
1995
期刊：
The American journal of physiology
影响因子：
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作者：
Perkins,SL;Kling,SJ
通讯作者：
Kling,SJ