Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry
Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry
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DOI：
10.4161/auto.7.9.16252
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发表时间：
2011-09-01
期刊：
影响因子：
13.3
通讯作者：
Albert, Matthew L.
中科院分区：
文献类型：
--
作者：
de la Calle, Claire;Joubert, Pierre-Emmanuel;Albert, Matthew L.
多种应激途径导致自噬和凋亡的诱导。目前的方法（例如，蛋白质凝胶印迹，显微镜）不能提供定量的单细胞分辨率，因此难以辨别这些途径是否相互排斥，或者在某些情况下，在执行细胞死亡时是否协同。我们报告了一种新的方法，使高通量，高内容的评估LC 3斑点和caspase-3切割在单细胞水平。
Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.