The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis
The auxin-regulated AP2/EREBP gene PUCHI is required for morphogenesis in the early lateral root primordium of Arabidopsis
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DOI：
10.1105/tpc.107.050674
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发表时间：
2007-07-01
期刊：
影响因子：
11.6
通讯作者：
Tasaka, Masao
中科院分区：
文献类型：
--
作者：
Hirota, Atsuko;Kato, Takehide;Tasaka, Masao
器官原基从创始细胞发育成器官是由于细胞分裂的协调模式。然而，在器官形成过程中，模式化的细胞分裂是如何被调节的，还没有很好的理解。在这里，我们表明，PUCHI基因，它编码一个假定的APETALA 2/乙烯响应元件结合蛋白转录因子，是所需的协调模式的细胞分裂过程中侧根形成拟南芥。PUCHI的隐性突变扰乱了侧根原基的细胞分裂模式，导致侧根近端区域肿胀。PUCHI表达最初在早期侧根原基的所有细胞中检测到，后来它被限制在原基的近端区域。PUCHI的稳定表达需要在其启动子区的生长素响应元件，外源生长素增加PUCHI mRNA的积累水平。这些结果表明，PUCHI行为下游的生长素信号，该基因有助于侧根形态发生，通过影响细胞分裂的模式在原基发育的早期阶段。
Organ primordia develop from founder cells into organs due to coordinated patterns of cell division. How patterned cell division is regulated during organ formation, however, is not well understood. Here, we show that the PUCHI gene, which encodes a putative APETALA2/ethylene- responsive element binding protein transcription factor, is required for the coordinated pattern of cell divisions during lateral root formation in Arabidopsis thaliana. Recessive mutations in PUCHI disturbed cell division patterns in the lateral root primordium, resulting in swelling of the proximal region of lateral roots. PUCHI expression was initially detected in all of the cells in early lateral root primordia, and later it was restricted to the proximal region of the primordia. Stable expression of PUCHI required auxin-responsive elements in its promoter region, and exogenous auxin increased the level of PUCHI mRNA accumulation. These results suggest that PUCHI acts downstream of auxin signaling and that this gene contributes to lateral root morphogenesis through affecting the pattern of cell divisions during the early stages of primordium development.