Single-cell infrared vibrational analysis by optical trapping mid-infrared photothermal microscopy
Single-cell infrared vibrational analysis by optical trapping mid-infrared photothermal microscopy
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DOI：
10.1039/d2an01917e
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发表时间：
2023-02-17
期刊：
影响因子：
4.2
通讯作者：
Tanaka, Takuo
中科院分区：
文献类型：
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作者：
Kato, Ryo;Yano, Taka-aki;Tanaka, Takuo
通过振动光谱结合光学捕获的单细胞分析是揭示大量群体中细胞间异质性的可靠平台。尽管红外振动光谱以无标记的方式提供了生物样品上丰富的分子指纹信息，但由于衍射限制聚焦的红外光束产生的弱梯度力和强的水吸收背景，其与光学捕获的应用从未实现。在这里，我们提出了单细胞红外振动分析，结合中红外光热（MIP）显微镜与光学捕获。光学捕获的单个聚合物颗粒和血液中的红细胞（RBC）可以通过它们的红外振动指纹进行化学鉴别。这种单细胞IR振动分析进一步使我们能够探测源自细胞内特征变化的RBC的化学异质性。我们的演示铺平了道路的红外振动分析的单细胞和化学表征在各个领域。
Single-cell analysis by means of vibrational spectroscopy combined with optical trapping is a reliable platform for unveiling cell-to-cell heterogeneities in vast populations. Although infrared (IR) vibrational spectroscopy provides rich molecular fingerprint information on biological samples in a label-free manner, its application with optical trapping has never been achieved due to weak gradient forces generated by the diffraction-limited focused IR beam and strong background of water absorption. Herein, we present single-cell IR vibrational analysis that incorporates mid-infrared photothermal (MIP) microscopy with optical trapping. Optically trapped single polymer particles and red blood cells (RBCs) in blood could be chemically identified owing to their IR vibrational fingerprints. This single-cell IR vibrational analysis further allowed us to probe the chemical heterogeneities of RBCs originating from the variation in the intracellular characteristics. Our demonstration paves the way for the IR vibrational analysis of single cells and chemical characterization in various fields.