Ganglion cell regeneration following whole-retina destruction in zebrafish
Ganglion cell regeneration following whole-retina destruction in zebrafish
复制标题
DOI：
10.1002/dneu.20568
复制
发表时间：
2008-02-01
影响因子：
3
通讯作者：
Stenkamp, Deborah L.
中科院分区：
文献类型：
--
作者：
Sherpa, Tshering;Fimbel, Shane M.;Stenkamp, Deborah L.
成年硬骨鱼的视网膜可以在损伤后再生神经元。目前的研究提供了第一个文件的功能性全视网膜再生的斑马鱼，Danio rerio，眼内注射细胞毒素，哇巴因。通过分析细胞死亡和细胞增殖以及通过分析视网膜特异性基因表达模式来监测层压视网膜组织的损失和替换。视网膜神经节细胞（RGC）再生的时空过程是通过使用选择性标记物，并发现在很大程度上重演胚胎神经节细胞神经发生的时空过程，在一个更长的时间框架。然而，没有观察到一些神经节细胞标志物的重新表达。通过测量视神经乳头（ONH）评价神经节细胞轴突的生长和寻路，发现正常ONH大小的恢复对应于两种视觉介导行为的恢复时间。然而，注意到一些异常，包括RGC的过度产生，以及在较长的恢复时间内ONH的进行性和过度生长。这个模型系统的全视网膜再生提供了一个信息的再生过程。(c)2007 Wiley Periodicals，Inc.
The retinas of adult teleost fish can regenerate neurons following injury. The current study provides the first documentation of functional whole retina regeneration in the zebrafish, Danio rerio, following intraocular injection of the cytotoxin, ouabain. Loss and replacement of laminated retinal tissue was monitored by analysis of cell death and cell proliferation, and by analysis of retina-specific gene expression patterns. The spatiotemporal process of retinal ganglion cell (RGC) regeneration was followed through the use of selective markers, and was found to largely recapitulate the spatiotemporal process of embryonic ganglion cell neurogenesis, over a more protracted time frame. However, the re-expression of some ganglion cell markers was not observed. The growth and pathfinding of ganglion cell axons was evaluated by measurement of the optic nerve head (ONH), and the restoration of normal ONH size was found to correspond to the time of recovery of two visually-mediated behaviors. However, some abnormalities were noted, including overproduction of RGCs, and progressive and excessive growth of the ONH at longer recovery times. This model system for whole-retina regeneration has provided an informative view of the regenerative process. (c) 2007 Wiley Periodicals, Inc.