Physical and functional interactions between hnRNP K and PRMT family proteins
Physical and functional interactions between hnRNP K and PRMT family proteins
复制标题
DOI：
10.1016/j.febslet.2008.12.025
复制
发表时间：
2009-01-22
期刊：
影响因子：
3.5
通讯作者：
Huang, Shih-Ming
中科院分区：
文献类型：
--
作者：
Chan, James Yi-Hsin;Hsieh, Tsai-Yuan;Huang, Shih-Ming
hnRNP - K和PRMT家族蛋白相互作用的机制尚不清楚。我们检查并证实了hnRNP K蛋白的精氨酸甲基化是由PRMT1，而不是CARM1通过它们的直接结合进行的。我们还使用共免疫沉淀分析研究了含有CARM1和PRMT1的hnRNP - K蛋白复合物。PRMT家族蛋白可能参与hnRNP - K在核受体辅激活、反激活、p21基因和蛋白表达中的功能调控。我们相信这些观察结果将有助于通过募集hnRNP - K蛋白相关蛋白（包括精氨酸甲基化修饰蛋白）来了解hnRNP - K蛋白功能的调控。
The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins.