A parametric study of electroacupuncture on persistent hyperalgesia and Fos protein expression in rats
A parametric study of electroacupuncture on persistent hyperalgesia and Fos protein expression in rats
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DOI：
10.1016/j.brainres.2004.01.092
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发表时间：
2004-09-10
期刊：
影响因子：
2.9
通讯作者：
Ren, K
中科院分区：
文献类型：
--
作者：
Lao, LX;Zhang, RX;Ren, K
我们先前报道了电针对无限制、未镇静和清醒大鼠模型持续性炎性疼痛的抗痛觉过敏作用。使用这个模型，诱导注射完全弗氏佐剂（CFA）到一个后爪，我们系统地评估了抗痛觉过敏的EA刺激参数（频率，强度，治疗持续时间，和脉冲宽度）。我们评估痛觉过敏的爪子缩回潜伏期（PWL）的有害的热刺激，发现10-和100-Hz的EA频率在电流强度为3 mA产生最大的抗痛觉过敏，当与其他参数相比。两种频率均显著增加早期痛敏的PWL（2.5和24 h-1 p < 0.05），10 Hz电针也显著增加后期的PWL（5 - 7 d; p < 0.05）。足够但可耐受的3 mA强度比较低强度（1-2 mA）更有效。20分钟的治疗比更长和更短（10和30分钟）的治疗产生更好的抗痛觉过敏。穴位特异性研究表明，电针足三里穴可产生明显的抗痛敏作用，而电针外关穴和足三里腹侧的假穴则无此作用。脊髓Fos蛋白表达的研究表明，最佳的EA选择性地抑制Fos的表达在浅层（I/II）和激活它在深层（III/IV）的脊髓背角。提示电针抗痛敏作用具有参数依赖性和穴位特异性，为进一步设计针刺治疗持续性炎性痛的临床研究提供了重要信息。(C)2004 Elsevier B. V.保留所有权利。
We previously reported the anti-hyperalgesia of electroacupuncture (EA) on persistent inflammatory pain in an unrestrained, unsedated, and conscious rat model. Using this model, induced by injecting complete Freund's adjuvant (CFA) into one hind paw, we systematically evaluated the anti-hyperalgesia of EA stimulation parameters (frequency, intensity, treatment duration, and pulse width). We assessed hyperalgesia by paw withdrawal latency (PWL) to a noxious thermal stimulus and found that 10- and 100-Hz EA frequencies at a current intensity of 3 mA produced the greatest anti-hyperalgesia, when compared to other parameters. Both frequencies significantly increased PWL in the early phases of hyperalgesia (2.5 and 24 h-1 p < 0.05), and 10 Hz EA also significantly increased PWL in later phases (5 to 7 days; p < 0.05). A sufficient but tolerable intensity of 3 mA was more effective than lower intensities (1-2 mA). A 20-min treatment produced better anti-hyperalgesia than longer and shorter (10 and 30 min) treatments. Acupoint specificity study demonstrated that GB30 produced significant EA anti-hyperalgesia, while Waiguan (TE5) and sham points, an abdominal point and a point at the opposite aspect of GB30, did not. The spinal Fos protein expression study demonstrated that the optimal EA selectively suppressed Fos expression in superficial laminae (I/II) and activated it in deeper laminae (III/IV) of the spinal dorsal horn. The results suggest that the EA anti-hyperalgesia is parameterdependent and point-specific, and they provide important information for designing further clinical acupuncture research on persistent inflammatory pain. (C) 2004 Elsevier B.V. All rights reserved.