Retrovirus-specific packaging of aminoacyl-tRNA synthetases with cognate primer tRNAs
Retrovirus-specific packaging of aminoacyl-tRNA synthetases with cognate primer tRNAs
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DOI：
10.1128/jvi.76.24.13111-13115.2002
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发表时间：
2002-12-01
影响因子：
5.4
通讯作者：
Kleiman, L
中科院分区：
文献类型：
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作者：
Cen, S;Javanbakht, H;Kleiman, L
在人类免疫缺陷病毒1型（HIV-1）、劳斯肉瘤病毒（RSV）和莫尼小鼠白血病病毒（Mo-MuLV）中，用于启动逆转录的tRNA分别是tRNA(3)（Lys）、tRNA（TrP）和tRNA（Pro）。通过对三种同源的人氨酰基- trna合成酶的抗体，我们发现HIV-1中只存在赖氨酸- trna合成酶（LysRS）， RSV中只存在色氨酸- trna合成酶（TrpRS），而Mo-MuLV中既不存在这两种合成酶，也不存在脯氨酸- trna合成酶（ProRS）。在HIV-1和RSV中，LysRS和trpr分别以大约25和12个分子/病毒粒子的形式存在。这些结果支持了HIV-1和RSV中同源的氨基酰基trna合成酶可能作为靶向选择性包装引物trna进入逆转录病毒的信号的假设。在Mo-MuLV中缺乏Pro，这与有报道称该病毒中tRNA（Pro）的选择性包装对于实现引物到Mo-MuLV基因组RNA的最佳退火不太重要相一致。
The tRNAs used to prime reverse transcription in human immunodeficiency virus type 1 (HIV-1), Rous sarcoma virus (RSV), and Moloney murine leukemia virus (Mo-MuLV) are tRNA(3)(Lys), tRNA(TrP), and tRNA(Pro), respectively. Using antibodies to the three cognate human aminoacyl-tRNA synthetases, we found that only lysyl-tRNA synthetase (LysRS) is present in HIV-1, only tryptophanyl-tRNA synthetase (TrpRS) is present in RSV, and neither these two synthetases nor prolyl-tRNA synthetase (ProRS) is present in Mo-MuLV. LysRS and TrpRS are present in HIV-1 and RSV at approximately 25 and 12 molecules/virion, respectively. These results support the hypothesis that, in HIV-1 and RSV, the cognate aminoacyl-tRNA synthetase may be used as the signal for targeting the selective packaging of primer tRNAs into retroviruses. The absence of ProRS in Mo-MuLV is consistent with reports that selective packaging of tRNA(Pro) in this virus is less important for achieving optimum annealing of the primer to Mo-MuLV genomic RNA.