Noninvasive fluorescence resonance energy transfer imaging of in vivo premature drug release from polymeric nanoparticles.
Noninvasive fluorescence resonance energy transfer imaging of in vivo premature drug release from polymeric nanoparticles.
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DOI：
10.1021/mp4002393
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发表时间：
2013-11-04
影响因子：
4.9
通讯作者：
Sun D
中科院分区：
文献类型：
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作者：
Zou P;Chen H;Paholak HJ;Sun D
了解体内药物释放动力学对于开发基于纳米颗粒的递送系统至关重要。在这项研究中，我们开发了一种荧光共振能量转移（FRET）成像方法，用于无创监测聚合物纳米颗粒的体外和体内货物释放。 FRET 供体染料（DiO 或 DiD）和受体染料（DiI 或 DiR）分别封装到聚（环氧乙烷）-b-聚苯乙烯（PEO-PS）纳米颗粒中。当 DiO（供体）纳米颗粒和 DiI（受体）纳米颗粒与癌细胞共孵育 2 小时时，从细胞膜观察到 FRET 信号增加，表明 DiO 和 DiI 快速释放到细胞膜。同样，静脉注射 DiD（供体）纳米颗粒和 DiR（受体）纳米颗粒后，在裸鼠中检测到 FRET 比率增加。相比之下，另一组裸鼠静脉注射。给予 DiD/DiR 共负载纳米颗粒后，FRET 比率降低。根据两组之间 FRET 比率的差异，确定 PEO-PS 纳米颗粒体内 DiD/DiR 释放半衰期为 9.2 分钟。此外，还观察到细胞膜的存在促进了亲脂性物质的爆发释放，而将油酸包覆的氧化铁掺入 PEO-PS 纳米颗粒中则减缓了 DiD/DiR 向细胞膜的释放。开发的体外和体内 FRET 成像技术可用于筛选用于亲脂性药物输送的稳定纳米制剂。
Understanding in vivo drug release kinetics is critical for the development of nanoparticle-based delivery systems. In this study, we developed a fluorescence resonance energy transfer (FRET) imaging approach to noninvasively monitor in vitro and in vivo cargo release from polymeric nanoparticles. The FRET donor dye (DiO or DiD) and acceptor dye (DiI or DiR) were individually encapsulated into poly(ethylene oxide)-b-polystyrene (PEO-PS) nanoparticles. When DiO (donor) nanoparticles and DiI (acceptor) nanoparticles were co-incubated with cancer cells for 2 h, increased FRET signals were observed from cell membranes, suggesting rapid release of DiO and DiI to cell membranes. Similarly, increased FRET ratios were detected in nude mice after intravenous co-administration of DiD (donor) nanoparticles and DiR (acceptor) nanoparticles. In contrast, another group of nude mice i.v. administrated with DiD/DiR co-loaded nanoparticles showed decreased FRET ratios. Based on the difference in FRET ratios between the two groups, in vivo DiD/DiR release half-life from PEO-PS nanoparticles was determined to be 9.2 min. In addition, it was observed that the presence of cell membranes facilitated burst release of lipophilic cargos while incorporation of oleic acid-coated iron oxide into PEO-PS nanoparticles slowed the release of DiD/DiR to cell membranes. The developed in vitro and in vivo FRET imaging techniques can be used to screening stable nano-formulations for lipophilic drug delivery.
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影响因子：
16.6
作者：
Li, Yuanpei;Xiao, Wenwu;Xiao, Kai;Berti, Lorenzo;Luo, Juntao;Tseng, Harry P.;Fung, Gabriel;Lam, Kit S.
通讯作者：
Lam, Kit S.
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通讯作者：
StOnge, G
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影响因子：
8.8
作者：
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