InhA1, NprA, and HlyII as Candidates for Markers To Differentiate Pathogenic from Nonpathogenic Bacillus cereus Strains
InhA1, NprA, and HlyII as Candidates for Markers To Differentiate Pathogenic from Nonpathogenic Bacillus cereus Strains
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DOI：
10.1128/jcm.02123-09
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发表时间：
2010-04-01
影响因子：
9.4
通讯作者：
Ramarao, Nalini
中科院分区：
文献类型：
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作者：
Cadot, Celine;Tran, Seav-Ly;Ramarao, Nalini
蜡样芽孢杆菌存在于食物、土壤和植物中，引起食源性疾病和机会性感染的能力可能因菌株而异。因此，除了检测细菌本身之外，测量有害毒素的产生可能是食品和医院安全的关键。所有以前的研究都集中在主要的已知毒力因子，cereulide，Hbl，Nhe和CytK。我们研究了其他毒力因子是否可能是致病菌株所特有的。InhA1、NprA和HlyII被描述为可能导致B。蜡状菌致病性我们报告了这三个新的毒力因子基因在57个B中的流行率和表达谱。从各种来源分离出的蜡样菌菌株，包括与胃肠道和非胃肠道疾病相关的菌株。使用PCR，定量逆转录酶PCR和体内毒力测定，我们解开了这些因素作为潜在的标记，以区分致病性和非致病性菌株。我们发现，hlyII基因携带的菌株具有致病潜力，inhA1和nprA的表达水平是在致病性比在非致病性组的菌株研究。这些数据提供了有关各种B致病性的有用信息。蜡状芽孢杆菌菌株。
Bacillus cereus is found in food, soil, and plants, and the ability to cause food-borne diseases and opportunistic infection presumably varies among strains. Therefore, measuring harmful toxin production, in addition to the detection of the bacterium itself, may be key for food and hospital safety purposes. All previous studies have focused on the main known virulence factors, cereulide, Hbl, Nhe, and CytK. We examined whether other virulence factors may be specific to pathogenic strains. InhA1, NprA, and HlyII have been described as possibly contributing to B. cereus pathogenicity. We report the prevalence and expression profiles of these three new virulence factor genes among 57 B. cereus strains isolated from various sources, including isolates associated with gastrointestinal and nongastrointestinal diseases. Using PCR, quantitative reverse transcriptase PCR, and virulence in vivo assays, we unraveled these factors as potential markers to differentiate pathogenic from nonpathogenic strains. We show that the hlyII gene is carried only by strains with a pathogenic potential and that the expression levels of inhA1 and nprA are higher in the pathogenic than in the nonpathogenic group of strains studied. These data deliver useful information about the pathogenicity of various B. cereus strains.