Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
Rif1 promotes association of G-quadruplex (G4) by its specific G4 binding and oligomerization activities
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DOI：
10.1038/s41598-019-44736-9
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发表时间：
2019-06-13
影响因子：
4.6
通讯作者：
Nagasawa, Kazuo
中科院分区：
文献类型：
--
作者：
Masai, Hisao;Fukatsu, Rino;Nagasawa, Kazuo
Rif1 是一种调节复制时间的保守蛋白，并优先结合裂殖酵母中的晚发/休眠起点附近。裂殖酵母基因组上的 Rif1 结合位点具有生成 G 四链体 (G4) 结构的内在潜力，纯化的 Rif1 优先结合该结构。我们之前提出，Rif1 生成的染色质结构可以通过促进染色质环的形成来确定复制时间。在这里，我们对 Rif1 及其 G4 结合进行了详细的生化分析。 Rif1 更喜欢含有长链鸟嘌呤的序列，并优先结合平行或杂合/混合拓扑的多聚体 G4。 Rif1 形成寡聚体并同时与多个 G4 结合。我们提出了一个模型，说明 Rif1 如何通过其 G4 结合和寡聚特性促进染色质结构的形成。
Rif1 is a conserved protein regulating replication timing and binds preferentially to the vicinity of late-firing/dormant origins in fission yeast. The Rif1 binding sites on the fission yeast genome have an intrinsic potential to generate G-quadruplex (G4) structures to which purified Rif1 preferentially binds. We previously proposed that Rif1 generates chromatin architecture that may determine replication timing by facilitating the chromatin loop formation. Here, we conducted detailed biochemical analyses on Rif1 and its G4 binding. Rif1 prefers sequences containing long stretches of guanines and binds preferentially to the multimeric G4 of parallel or hybrid/mix topology. Rif1 forms oligomers and binds simultaneously to multiple G4. We present a model on how Rif1 may facilitate the formation of chromatin architecture through its G4 binding and oligomerization properties.