Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression
Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression
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DOI：
10.1101/2020.02.09.940601
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发表时间：
2020-02
影响因子：
4
通讯作者：
Peter J. Taylor;J. Hagen;F. Faruqu;K. Al‐Jamal;Bonnie Quigley;M. Beeby;M. Selkirk;P. Sarkies
中科院分区：
文献类型：
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作者：
Peter J. Taylor;J. Hagen;F. Faruqu;K. Al‐Jamal;Bonnie Quigley;M. Beeby;M. Selkirk;P. Sarkies
许多生物体，包括寄生线虫，分泌小RNA到细胞外环境中，大部分封装在小囊泡中。寄生虫分泌的物质通常含有microRNA（miRNAs），这增加了它们可能通过调节靶细胞中的宿主基因而导致病理学的可能性。在这里，我们将材料从寄生线虫旋毛虫在两个不同的生命阶段。我们发现成年T.寄生在肠粘膜中的螺旋体在囊泡内分泌miRNA。但出乎意料的是，T。螺旋肌期幼虫（MSL），其在骨骼肌细胞内细胞内生活，分泌似乎未被封装的miRNA。值得注意的是，分泌的miRNA包括哺乳动物miRNA-31的同源物，其在肌肉发育中具有重要作用。因此，我们的工作提出了一个新的潜在的RNA分泌机制与T。螺旋体感染
Many organisms, including parasitic nematodes, secrete small RNAs into the extracellular environment largely encapsulated within small vesicles. Parasite secreted material often contains microRNAs (miRNAs), raising the possibility that they might contribute to pathology by regulating host genes in target cells. Here we characterise material from the parasitic nematode Trichinella spiralis at two different life stages. We show that adult T. spiralis, which inhabit intestinal mucosa, secrete miRNAs within vesicles. Unexpectedly however, T. spiralis muscle stage larvae (MSL), which live intracellularly within skeletal muscle cells, secrete miRNAs that appear not to be encapsulated. Notably, secreted miRNAs include a homologue of mammalian miRNA-31, which has an important role in muscle development. Our work therefore suggests a new potential mechanism of RNA secretion with implications for the pathology of T. spiralis infection.