MODIFICATION OF GONADECTOMY-INDUCED INCREASES IN BRAIN MONOAMINE METABOLISM BY STEROID-HORMONES IN MALE AND FEMALE RATS
MODIFICATION OF GONADECTOMY-INDUCED INCREASES IN BRAIN MONOAMINE METABOLISM BY STEROID-HORMONES IN MALE AND FEMALE RATS
复制标题
DOI：
10.1016/0306-4530(91)90038-u
复制
发表时间：
1991-01-01
影响因子：
3.7
通讯作者：
SHAPIRO, BH
中科院分区：
文献类型：
--
作者：
BITAR, MS;OTA, M;SHAPIRO, BH
在慢性性腺切除、类固醇或油处理的雄性和雌性大鼠的选定脑区中测量单胺（多巴胺、DA、5-羟色胺）及其主要代谢产物（高香草酸- HVA、二羟基苯乙酸- DOPAC、5-羟基吲哚乙酸- 5-HIAA）的浓度。DOPAC和HVA的浓度显着增加下丘脑（男性，女性），纹状体（男性，女性）和脑干（男性）性腺切除术后，而DA的水平保持不变，在大多数的大脑区域检查。大多数的变化被逆转或减弱慢性雌二醇（EB）替代。与此相反，生理浓度的睾酮（TP）的慢性治疗减少DA营业额的指标，仅在纹状体的卵巢切除（OVX）和脑干的卵巢切除（RDX）大鼠。与ORDX相关的纹状体DOPAC和HVA水平的增加并没有被EB或TP逆转。使下丘脑、纹状体5-HIAA含量增加，使下丘脑、海马5-HT含量减少。这些变化被逆转的TP或EB的慢性治疗。去卵巢后脑内5-HT代谢无明显改变，而去性腺和长期类固醇替代治疗可改变脑内单胺代谢，且具有脑区和性别依赖性。我们的数据表明，在雄性和雌性大鼠的大脑单胺能神经元的活性性腺切除相关的增加衰减更有效地与生理浓度的雌二醇比睾酮。许多脑区的单胺能神经元不敏感（例如，下丘脑、纹状体）对睾酮作用的影响在两种性别中都很明显。
Concentrations of monoamines (dopamine, DA; serotonin, 5-HT) and their major metabolites (homovanillic acid - HVA; dihydroxyphenylacetic acid - DOPAC; 5-hydroxyindolacetic acid - 5-HIAA) were measured in selected brain areas of chronically gonadectomized, steroid- or oil-treated male and female rats. Concentrations of DOPAC and HVA were markedly increased in the hypothalamus (male, female), striatum (male, female) and brainstem (male) following gonadectomy, whereas the levels of DA remained unaltered in most of the brain areas examined. Most of the changes were reversed or attenuated by chronic estradiol (EB) substitution. In contrast, chronic treatment with physiological concentrations of testosterone (TP) reduced indexes of DA turnover only in the striatum of ovariectomized (OVX) and brainstem of orchidectomized (ORDX) rats. ORDX-related increases in striatal levels of DOPAC and HVA were not reversed by either EB or TP. ORDX increased the levels of 5-HIAA (hypothalamus, striatum) and decreased those of 5-HT (hypothalamus, hippocampus). These changes were reversed by chronic treatment with either TP or EB. Brain metabolism of 5-HT remained unaltered following OVX.Gonadectomy and chronic steroid replacement therapy appear to alter brain monoamine metabolism in a brain region and sex-dependent manner. Our data demonstrate that gonadectomy-related increases in the activity of brain monoaminergic neurons in both male and female rats was attenuated more effectively with physiological concentrations of estradiol than with testosterone. Insensitivity of monoaminergic neurons in a number of brain areas (e.g., hypothalamus, striatum) to the action of testosterone was evident in both sexes.